Meiji Seika Kaisha, Ltd.

National Institute of Advanced Industrial Science and Technology

Chuhei Nojiri

Yujiro Yamada

Taro Niida

Takashi Shomura

Tomizo Niwa

Norio Ezaki

Shinji Miyado

Takashi Tsuruoka

Shigeharu Inouye

Koji Nishizawa

Shigeo Seki

Kaoru Okakura

Fumio Kai

Yasuaki Ogawa

Takashi Yamanobe

Kazunori Ohba

Osamu Makabe

Takeo Nomura

Hiroyuki Hamaguchi

Shoichi Amano

Jiro Itoh

Kozo Nagaoka

Hitoshi Goi

Shinichiro Kuriya

Tomoyuki Fukasawa

Keita Matsunaga

Nobuo Matsuhashi

Kiyoyuki Ogata

Kunikazu Totsugawa

Mitsugu Ito

Hiromi Toyama

The invention is to provide a novel &#x3b2;-glucosidase and a gene that codes for the enzyme, and to develop a technique of utilizing the &#x3b2;-glucosidase or a composition that contains the enzyme for processing plants or plant-derived substances. According to the invention, there are provided a novel enzyme showing a &#x3b2;-glucosidase activity and derived from filamentous fungi of the genus Acremonium, a gene that codes for the enzyme, a method of using the gene for expressing &#x3b2;-glucosidase, an enzyme composition that contains &#x3b2;-glucosidase, and a method of processing plants or plant-derived substances with the enzyme or the enzyme compositions.

Enzyme having &#x3b2;-glucosidase activity and use thereof

Megakaryocyte colony stimulating factor and process for its preparation

Essentially pure plasmids derived from Actinomycetes are described which have at least one restriction cleavage site for specific restriction enzyme and have molecular weight of less than 1.0.times.10.sup.7.

Plasmids derived from Actinomycetes

New antibiotics, SF-2050 substance and SF-2050B substance are produced by cultivating a microorganism Streptomyces sp. SF-2050 now deposited under FERM-P 4358 and under ATCC. 31450 in a liquid culture medium under aerobic conditions, and these antibiotics may be isolated from the fermentation broth and useful as an antibacterial agent. These antibiotics have an activity inhibitory to .beta.-lactamase.

Antibiotics SF-2050 and SF-2050B substances and production and use

Compounds are provided having the general formula I: ##STR1## wherein X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue. The formula I compounds are prepared from compounds of the general formula II: ##STR2## wherein R.sub.1 represents hydrogen, a lower alkanoyl group, an arylalkanoyl group, an alkoxycarbonyl group, a lower haloalkoxycarbonyl group, a substituted or unsubstituted aroyl group, a N-arylcarbamoyl group of a substituted or unsubstituted aryl group; R.sub.2 either represents hydrogen or, together with the ##STR3## group, represents a phthalimido group; and X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue, or their metal salts or their salts with organic bases, by causing the culture of a cephalosporin C acylase-producing strain of a microorganism, especially a mold, fungus, or the secondary preparation therefrom, to act on the latter compounds II in the presence of an aqueous medium.

Process for the preparation of 7-amino-cephem compounds using mold fungi

A new antibiotic designated BN-130 substance is now provided, which has antibacterial activity against gram-negative and gram-positive bacteria as well as acid-fast bacteria. BN-130 substance is produced by cultivating a strain of Pseudomanas stutzeri under aerobic conditions.

Antibiotic BN-130 substances and the production thereof

Growing community of inventors

Yokohama, Japan

Chuhei Nojiri