Peking University

Institute of Radiation Medicine Academy of Military Medical Science

Beijing Sanly Sci-tech Develop Inc. Ltd.

Institute of Radiation Medicine, Academy of Military Medical Sciences, Pla.

Chuanling Zhang

Lihe Zhang

Demin Zhou

Sulong Xiao

Longlong Si

Fei Yu

Qing Xia

Zuze Wu

Zhongxiong Tang

Huan Xu

Aiping Yu

Tianzhuo Yao

Yongxiang Zheng

Xueying Zhou

Ziwei Zhang

Zhenyu Tian

The present invention relates to a mutated virus. Said virus can be an influenza virus of human or other animal origin. The present invention also relates to a method for preparing the mutated virus, the method comprising introducing UAG codons into positions upstream of the stop codons per se of one or more genes of a viral genome by reverse genetic techniques. The present invention further relates to uses of the mutated virus, for example, as a live attenuated vaccine, or in replication of controllable and safe virus models, and the like.

Mutant virus, preparation method therefor and application thereof

The present invention relates to an adeno-associated virus with site-directed mutagenesis and site-specific modification, and a preparation method and uses thereof. Specifically, the present invention uses genetic code expansion techniques to incorporate non-natural amino acid into an adeno-associated virus capsid protein VP1 or fragment thereof, thereby obtaining an adeno-associated virus with site-directed mutagenesis using the non-natural amino acid. The adeno-associated virus with site-directed mutagenesis is equivalent to a wild-type virus in terms of production, transduction and mobility, can couple with other functional molecules, such as targeting molecules, and can carry a functional gene in a normal manner, which indicates that the adeno-associated virus with site-directed mutagenesis can be used as a tool adeno-associated virus, and applied in various aspects associated with adeno-associated virus such as finding adeno-associated virus binding proteins or using as target genetic therapy vector.

Adeno-associated virus with site-directed mutagenesis and site-directed modification and preparation method and application thereof

Provided is an anticoagulant fusion protein comprising oligopeptide recognizable and cleavable by either factor XIa and factor Xa or thrombin and factor Xa. Also provided are the preparation method of the anticoagulant fusion protein and medicinal use thereof.

Preparation of low bleeding anticoagulant fusion protein and its use

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Beijing, China

Chuanling Zhang