International Business Machines Corporation

University of Chicago

Epiplanta Biotech Ltd.

Pacific Biosciences of California, Inc.

Chuan He

Xiao Ling Chen

Yan Huang

Jiang Yi Liu

Jian Xu

Chong Zhou

Wei Wu

Guifang Jia

Chunxiao Song

Liang Zhang

Jonas Korlach

Tyson A Clark

Boxuan Simen Zhao

Wu Wei

Chang Liu

Pradnya Narkhede

Ji Nie

Xiao-Long Cui

Xiaolong Cui

Miao Yu

Lu Zhou

Qing Dai

Michael Bosscher

Xingyu Lu

Kai Chen

The methods, compositions, and kits of the disclosure provide a novel approach for a whole genome, unbiased DNA analysis method that can be performed on limited amounts of DNA. can be used to analyze DNA to determine its modification status. Aspects of the disclosure relate to a method for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules comprising: (a) ligating an adaptor to the DNA molecules, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA.

Methods for the amplification of bisulfite-treated DNA

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an mA demethylase is introduced, wherein said mA demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an mA demethylase into a regenerable plant cell, and regenerating a transgenic plant from the regenerable plant cell.

Transgenic plant and the method for producing the same

The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence is adenosine.

Composition and methods for detecting adenosine modifications

A system and method to verify software includes a debugger setting a breakpoint in the software. The breakpoint indicates a point at which to pause or stop execution of the software. The method also includes setting one or more anchor points associated with the breakpoint. Each of the one or more anchor points represents another point in the software that must be executed prior to pausing or stopping the execution of the software at the breakpoint.

Breakpoint with specified anchor points

Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.

Method for highly sensitive DNA methylation analysis

Aspects include detecting, by an agent of a remote debugging tool that a first controller currently associated with the agent for a debugging session has not responded to a status inquiry from the agent. The first controller interacts with an end user, sends requests to the agent to operate a target program, and processes responses from the agent. Based on detecting that the first controller has not responded to the status inquiry from the agent, the agent identifies a second controller, associates the second controller with the agent for the debugging session, and resumes the debugging session with the second controller in place of the first controller. The associating includes synchronizing a debugging session state between the second controller and the agent. The target program continues to execute during the identifying, associating, and resuming, and the debugging session state is not changed by the identifying, associating, and resuming.

Resuming a remote debugging session using a backup node

Embodiments of the present invention provide a method to quickly insert new rows or columns in a tabular data structure (such as a spreadsheet, or a table). The user expands a row height (or a column width). The expanded row (or column width) is automatically displayed with proposed divisions (for example, represented with dashed lines). The user indicates acceptance of the proposed divisions. The expanded row (or column) is automatically divided into multiple rows (or columns) as indicated by the proposed divisions.

Inserting new elements in a tabular data structure

A method, computer program product and/or system for pre-fetching disassembly code. A breakpoint is set within an application under test (AUT). Setting of the breakpoint triggers pre-fetching of disassembly code associated with the breakpoint. The pre-fetched disassembly code is retained in a store local to a debug analysis system. When runtime processing of the AUT reaches the breakpoint, the debug analysis system retrieves the disassembly code from the local store.

Pre-fetching disassembly code for remote software debugging

The present invention relates to methods and compositions for detecting, evaluating, and/or mapping 5-methyl-modified and/or 5-hydroxylmethyl-modified cytosine bases within a nucleic acid molecule.

Composition and methods related to modification of 5-methylcytosine (5-mC)

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Beijing, China

Chuan He