Industrial Technology Research Institute

Chuan-Chuan Huang

Min-Yuan Chou

Hsiu-Chuan Li

Chia-Yu Fan

Ya-Ping Lai

The present invention relates to a method for producing multivalent Fab fragments. In particular, the invention relates to a method for the generation of trimeric Fab fragments by co-expression of a gene construct comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide, and a gene construct consisting of a light chain portion of an IgG in mammalian cells. Uses of molecules generated using the method of the invention are also described.

Method for producing a multivalent fab fragment with collagen-like peptide

A collagen scaffold domain, including a collagenous or collagen-like domain, which directs self-trimerization is provided. The collagen scaffold domain can be fused to one or more heterologous domains, such as an antibody domain. Methods for generating and using the scaffold domains and fusion proteins are also provided.

Trimeric collagen scaffold antibodies

A DNA molecule consisting of the nucleotide sequence of SEQ ID NO: 1, which encodes a collagenous (COL1) domain and a C-terminal noncollagenous (NC1) domain of type XXI collagen. Expression systems and methods for the expression of the DNA molecule are also provided.

Growing community of inventors

Taipei County, Taiwan

Chuan-Chuan Huang