Micronics, Inc.

Visiongate, Inc.

Christy A Lancaster

Bernhard Hans Weigl

C Frederick Battrell

Jeffrey M Houkal

Mingchao Shen

Patrick Saltsman

Wayne L Breidford

Jon W Hayenga

Ronald L Bardell

Jeffrey F Tonn

Michael G Meyer

Mark Fauver

J Richard Rahn

Thomas Neumann

Anna V Tourovskaia

Julia Oi Yan Yu

A method for 3D imaging of a biologic object () in an optical tomography system where a subcellular structure of a biological object () is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object () is moved relatively to a microscope objective () to present varying angles of view and the labeled biological object () is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object () and the microscope objective () within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object () from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ().

Functional imaging of cells with optical projection tomography

The present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.

Microfluidic devices for fluid manipulation and analysis

An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.

System and method for heating, cooling and heat cycling on microfluidic device

A microfluidic system for isolation and amplification of DNA or RNA from aqueous solutions and detection of the DNA or RNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA or RNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA or RNA are transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays.

Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing

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Seattle, WA, United States of America

Christy A Lancaster