Codex Dna, Inc.

Synthetic Genomics, Inc.

Molecular Assemblies, Inc.

Christopher Wilson

Matthew T Weinstock

Eric D Hesek

J William Efcavitch

Julie L Tubbs

Prem Sinha

Boguslaw Stec

Deanne W Sammond

The present disclosure relates to the seminal discovery of a generation and use of genetically engineeredsp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery ofsp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.

[object Object]

The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3'-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.

Modified template-independent enzymes for polydeoxynucleotide synthesis

The present disclosure provides engineeredsp. organisms. The organisms can be engineered to have an altered Chromosome II so that the altered Chromosome II can be used insp. and other organisms for the cloning or amplification of nucleic acid molecules and for the expression and production of proteins and peptides in asp. organism. One or more genetic elements have been deleted from Chromosome II and/or relocated from Chromosome II to Chromosome I. The engineeredsp. organisms of the invention can also have signal sequences fused to proteins or peptides to be secreted from the cell. In some embodiments the engineeredsp. organisms can have sequences that enable them to retain viability after incubation at low temperatures.

Genetically engineered Vibrio sp. and uses thereof

Genetically engineeredsp. and uses thereof

Growing community of inventors

San Diego, CA, United States of America

Christopher Wilson