Luminex Corporation

Eragen Biosciences, Inc.

Christopher W Scherrer

James Robert Prudent

David J Marshall

Christopher B Sherrill

Jerod L Ptacin

Jennifer K Grenier

Craig S Richmond

Eric B Roesch

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.

Solid support assay systems and methods utilizing non-standard bases

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.

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Rio, WI, United States of America

Christopher W Scherrer