Massachusetts Institute of Technology

The General Hospital Corporation

The Broad Institute, Inc.

Christopher Smillie

Aviv Regev

Alexander K Shalek

Ramnik Xavier

Moshe Biton

Rebecca H Herbst

Jose Ordovas-Montanes

Orit Rozenblatt-Rosen

Karthik Shekhar

Adam Haber

Noga Rogel

An atlas of intestinal epithelial cells, intestinal epithelial stem cells and intestinal immune cells identifies new cell populations, markers, networks, and responses to stimuli. Intestinal T cells drive intestinal epithelial cell differentiation and activity. Accordingly, disclosed are methods of modulating intestinal epithelial cell differentiation, maintenance and/or function, related methods for the treatment of disease, including IBD. Also disclosed are methods and kits for identifying cell types, their differentiation, homeostasis and activation.

Modulation of intestinal epithelial cell differentiation, maintenance and/or function through T cell action

The present invention provides for a human cell atlas of the colon from healthy and diseased subjects. The atlas was obtained by single sequencing of about 117,000 cells. The present invention discloses novel markers for cell types. Moreover, genes associated with disease are identified in the colon and colon specific cell types. The invention provides for diagnostic assays based on gene markers and cell composition, as well as target cell types that express genes associated with disease. Finally, disclosed are novel cell types and methods of quantitating, detecting and isolating the cell types.

Cell atlas of the healthy and ulcerative colitis human colon

Growing community of inventors

Cambridge, MA, United States of America

Christopher Smillie