Pacific Biosciences of California, Inc.

Christopher Silk

Keith P Bjornson

John Lyle

Lei Jia

Sonya Clark

Mark Chaisson

Systems and methods are provided for single-molecule sequencing of template nucleic acids in which the signal from a label attached to a polymerase enzyme is used to monitor conformational changes in the polymerase which occur while labeled nucleotides or nucleotide analogs are added to a growing nucleic acid chain which is complementary to the template nucleic acid. The signal indicative of the conformational state of the enzyme is used to determine with higher confidence when true nucleotide or nucleotide analog incorporation events occur, allowing for the improved quality of base calls and sequence determination.

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Oakland, CA, United States of America

Christopher Silk