Texaco Inc.

Other

Ethyl Corporation

Christopher S Liu

Maria M Kapuscinski

Larry D Grina

William P Hart

Benjamin J Kaufman

Nicholas Benfaremo

Ronald E Jones

Theodore E Nalesnik

Harry Chafetz

Cyril A Migdal

Maria M Kapusciniski

Donald J Clarke

Rodney L Sung

Dennis E Hruby

Thomas F DeRosa

Joseph M Russo

Scott J Hultgren

Robert T Biggs

James R Ketcham

Brian L Papke

Mark D Levin

Christine A Franke

Scott O Lindholm

Roy Isamu Yamamota

Norris Roland Crawford

Thomas A Kennedy

Hal C Jones

Maria M Kapucinski

Constance A Gallagher

Amy K Evans

William A Buscher, Jr

The DegP (HtrA) protease is a multifunctional protein essential for the removal of misfolded and aggregated proteins in the periplasm. The present invention provides an assay for inhibitors of DegP activity, comprising mixing a suspected inhibitor of DegP activity with DegP and a suitable substrate (preferably a native substrate of DegP such as PapA) and detecting changes in DegP activity. DegP has been shown to be essential for virulence in several Gram negative pathogens. Only three natural targets for DegP have been described: colicin A lysis protein (Cal), pilin subunits (K88, K99, Pap) and recently HMW1 and HMW2 from,In vitro, DegP has shown weak protease activity on casein and several other non-native substrates. The present inventors have identified the major pilin subunit of the Pap pilus, PapA, as a native DegP substrate and demonstrated binding and proteolysis of this substrate in vitro. Using an NH,-terminal affinity tag the present inventors have purified PapA away from the PapD chaperone, in the presence of denaturant, to use as a proteolysis substrate. This finding will allow the identification of the DegP recognition and cleavage sites in substrate proteins, and further, allow the design of small molecule inhibitors of protease function.

DegP periplasmic protease a new anti-infective target and an in vitro assay for DegP protease function

A lubricating oil composition comprising:

Polyisobutylene succinimide and ethylene-propylene succinimide

A motor fuel additive composition comprising a mixture of an amido alkanolamine and a polyisobutylene amine is provided. A fuel composition providing intake valve and combustion chamber detergency is also provided.

Motor fuel detergent additives

Lube oils of improved properties contain ethylene-propylene copolymer bearing moieties derived from the reaction product of allyl glycidyl ether and a heterocyclic amine such as N-(3-aminopropyl) morpholine.

Lubricating oil containing dispersant viscosity index improver

Lubricating oil composition containing the reaction product of an

An additive composition comprising a graft and amine-derivatized copolymer prepared from ethylene and at least one C.sub.3 to C.sub.10 alpha-monoolefin and, optionally, a polyene selected from non-conjugated dienes and trienes comprising from about 15 to 80 mole percent of ethylene, from about 20 to 85 mole percent of said C.sub.3 to C.sub.10 alpha-monoolefin and from about 0 to 15 mole percent of said polyene, said copolymer having a number average molecular weight ranging from about 5,500 to 50,000 and having grafted thereon at least 1.8 molecules of a carboxylic acid acylating function per molecule of said copolymer and reacting said grafted copolymer with an amino-aromatic polyamine compound from the group consisting of an N-arylphenylenediamine, an aminocarbazole, an aminoindole, an amino-indazolinone, an aminomercaptotriazole, and an amino-perimidine to form said graft and amine-derivatized copolymer, and a lubricating oil composition containing same are provided.

Dispersant and antioxidant additive and lubricating oil composition

An additive composition prepared by reacting a polymer prepared from ethylene and at least one C.sub.3 -C.sub.10 alpha-monoolefin and optionally a polyene selected from non-conjugated dienes and trienes comprising from about 15 to 80 mole percent of ethylene from about 20 to 85 mole percent of said C.sub.3 -C.sub.10 alpha-monoolefin and from about 0 to 15 mole percent of said polyene and having an average molecular weight ranging from about 5,000 to 500,000 with a nitrosodiphenylamine compound represented by the formula: ##STR1## in which R and R.sup.1 represent hydrogen or an alkyl, alkenyl, or alkoxyl radical having from 1 to 8 carbon atoms, and a lubricating oil composition containing same is provided.

Dispersant anti-oxidant VI improver and lubricating oil composition

A lubricating oil contains, as a multifunctional additive, a polymer such as an ethylene - propylene copolymer, bearing graft moieties derived from allyl glycidyl ether, or glycidyl methacrylate as a graft monomer polymer being functionalized with (i) N-vinyl pyrrolidone and (ii) a Mannich base formed by the reaction p-nonyl phenol, N-(aminoethyl) piperazine, and formaldehyde.

Lubricating oil containing viscosity index improver

A lubricating oil contains a carbon-carbon backbone polymer containing residual unsaturation and bearing graft moieties derived from graft monomers containing ethylenic unsaturation and an epoxide moiety, and bonded to at least one of said graft moieties through an opened epoxide moiety, a Mannich base, prepared by reaction of an amine, an aldehyde, and a hindered phenol containing an active hydrogen atom.

Lubricating oil containing a Mannich base

A diesel fuel of improved flow properties at low temperatures which contains a linear, substantially oil-soluble polymer having a carbon-carbon backbone bering a graft moiety derived from, as a graft monomer, an unsaturated-hydrocarbon ester of an (C.sub.1 -C.sub.18) alkyl alcohol containing an ethylenically unsaturated carbon-carbon double bond.

Diesel fuel compositions containing polyolefin graft polymers

Lubricating oils of improved oxidative stability, dispersancy, and viscosity index contain ethylene-propylene copolymer bearing allyl glycidyl ether or glycidyl methacrylate graft monomer--which has been functionalized with 2,5-dimethyl aniline or 2,6-dimethyl morpholine.

Multifunctional viscosity index improver

An oil containing a viscosity index improver of improved oxidative stability contains, as an anti-oxidant system 2,6-di-t-butylphenol and 1-3-aminopropyl-2-pipecoline.

Anti-oxidant system

Lubricating oil of improved dispersancy and viscosity index contains ethylene-propylene polymer bearing glycidyl methacrylate graft which as been functionalized with mercaptoacetic acid.

Lubricating oil of improved dispersancy and anti-oxidancy contains an ethylene-propylene copolymer onto which has been graft polymerized allyl glycidyl ether, the graft polymer having been thereafter functionalized with (i) N-vinyl pyrrolidone and (ii) a Mannich base formed from N-(aminoethyl) piperazine, formaldehyde, and nonyl phenol.

An oil containing a viscosity index improver of improved oxidative stability contains, as an anti-oxidant system, 2,6-di-t-butylphenol and N-aminoethylpiperazine.

Growing community of inventors

Poughkeepsie, NY, United States of America

Christopher S Liu