Kosan Biosciences Incorporated

Christopher Reeves

Daniel T Chu

Chaitan Khosla

Daniel V Santi

Kai Wu

Andreas W Schirmer

Maria Fardis

Ralph C Reid

Bryan Julien

Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.

Polynucleotides encoding the fkbB gene of the FK-520 polyketide synthase gene cluster

Domains of ambruticin polyketide synthase and modification enzymes and polynucleotides encoding them are provided. Methods to prepare ambruticin in pharmaceutically useful quantities are described, as are methods to prepare ambruticin analogs and other polyketides using the polynucleotides encoding ambruticin synthase domains or modifying enzymes.

Biosynthetic gene cluster for ambruticins and the encoded proteins

Domains of jerangolid polyketide synthase and modification enzymes and polynucleotides encoding them are provided. Methods to prepare jerangolid in pharmaceutically useful quantities are described, as are methods to prepare jerangolid analogs and other polyketides using the polynucleotides encoding jerangolid synthase domains or modifying enzymes.

Biosynthetic gene cluster for jerangolids

Polyketide compounds of the formula but not including FK-506, FK-520, 18-hydroxy-FK520 and 18-hydroxy-FK-506.

Polyketide synthase enzymes and recombinant DNA constructs therefor

Polynucleotides encoding the fkbA gene of the FK-520 polyketide synthase gene cluster

Polyketide derivatives

Isolated nucleic acids relating to the fkbA gene within the FK-520 polyketide synthase gene cluster

Growing community of inventors

Orinda, CA, United States of America

Christopher Reeves