Temple University

Christopher R Yonan

Frank N Chang

A method of visualizing proteins bound to a protein-binding membrane is provided herein, comprising reacting proteins bound to protein-binding membranes with a compound of formula (I) wherein R, R, R, R, R, M, n, and m are as defined herein. 

Method of visualizing proteins bound to protein-binding membranes

Proteins can be rapidly separated to a high degree of resolution by electrophoresis on polymeric membranes that have high protein binding capacity. The electrophoretic separation is carried out in a low conductivity, water-miscible organic solvent buffer. The low conductivity of the organic solvent buffer minimizes heat generation, and the water-miscible nature of the organic solvent buffer permits the analysis of hydrophobic and low molecular weight proteins as well as hydrophilic proteins. When electrophoresis is conducted under non-denaturing conditions, it allows the detection of enzymatic activities, protein-protein interactions and protein-ligand interactions.

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Plymouth Meeting, PA, United States of America

Christopher R Yonan