Sterling Winthrop Inc.

Christopher P Phillips

Robert A Snow

Gary G Liversidge

Kenneth C Cundy

The present invention is directed to a method of labeling a protein conjugate with a radionuclide to form a radioconjugate comprising reacting: (a) the radionuclide; and (b) the protein conjugate in an acetate buffer for a time period and under conditions sufficient to form said radioconjugate. Preferably, the protein conjugate is comprised of a complexing agent moiety and a free epsilon amino group-containing protein moiety. The completing agent moiety is preferably a terpyridine analog. The terpyridine analog is preferably TMT. Preferably, the free epsilon amino group-containing protein moiety is an immunoreactive protein. The immunoreactive protein is preferably the monoclonal antibody ING-1. In a preferred embodiment, the protein conjugate is TMT-ING-1, and the radionuclide is .sup.90 Y. In a further preferred embodiment, the radioconjugate is chelated to greater than 98 percent of the radionuclide. Preferably, the acetate buffer in which the protein conjugate is present comprises at least about 50 mM sodium acetate.

Preparation of a radioconjugate formulation

Disclosed are lyophilized biologically active proteinaceous compositions containing low diol polyalkylene oxide, such as polyethylene glycol, covalently attached to a biologically active proteinaceous substance and combined with the cryoprotectant cyclodextrin.

Lyophilized polyethylene oxide modified protein and polypeptide

Lyophilized polyethylene oxide modified catalase composition,

This invention discloses a composition comprised of nanoparticles having a surface modifier adsorbed on the surface thereof and a cryoprotectant associated therewith, which cryoprotectant is present in an amount sufficient to form a nanoparticle-cryoprotectant composition. A preferred surface modifier is polyvinylpyrrolidone, and a preferred cryoprotectant is a carbohydrate such as sucrose. This invention further discloses a method of making nanoparticles having a surface modifier adsorbed on the surface and a cryoprotectant associated therewith, comprised of contacting said nanoparticles with the cryoprotectant for a time and under conditions sufficient to form a nanoparticle-cryoprotectant composition.

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Brandamore, PA, United States of America

Christopher P Phillips