Harvard College

Foster-miller, Inc.

Christopher Michael Rose

Steven P Gygi

Scott Paul Quigley

Brian K Erickson

John D Larouco

Joseph Vincent Malone

Robert A Everley

Michael Ian Andrewartha

Michael Vincent Salvucci

Steven Anthony Caito

Mark Radiwon

John Michael Reed

Frederick Carl Rickert Ii

Described herein is a kit of materials prepared for assays that involve determining relative abundance and/or absolute abundance of various targeted peptides. The kit may comprise trigger versions of target peptides with masses offset from the respective target peptides by predetermined and known amounts. The trigger peptides may be present in amounts that may be readily detected via a mass spectrometry analysis. When mixed with samples that are analyzed, detection of the trigger peptides indicates where in the mass-spectrometer output the target peptide may be found. The kit may include a predetermined amount of synthetic versions of one or more of the target peptides. A measured relative abundance of this synthetic peptide relative to that of the target peptides yields an absolute quantitative value of the target peptide. Also disclosed is a method of preparing a plurality of samples to be submitted for mass spectrometer analysis in parallel.

Techniques for high throughput targeted proteomic analysis and related systems and methods

Growing community of inventors

Fremont, CA, United States of America

Christopher Michael Rose