Cambridge Enterprise Limited

Cambridge University Technical Services Limited

Harvard College

Wren Therapeutics Limited

Christopher Martin Dobson

Tuomas Knowles

Jesus Zurdo

Fabrizio Chiti

Michele Vendruscolo

Therese Herling

Emma Yates

Tuomas Pertti Jonathan Knowles

Kateri Hayashi DuBay

Samuel Cohen

Luke Rajah

Duncan White

David A Weitz

Pavan Kumar Challa

Julius Bier Kirkegaard

Quentin Alexis Eric Peter

Johnny Habchi

Sean Chia

Ulyana Shimanovich

Tadas Kartanas

Michele Perni

Pietro Sormanni

Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.

Fluidic separation and detection

The present invention provides capsules having a shell of material that comprises an assembly of a protein, and the capsule is optionally provided with a network of material within the shell that is an assembly of the protein. The assembly of the protein is obtained or obtainable by the aggregation of the protein, optionally together with another protein. The assembly is a non-covalent assembly of a protein.

Protein capsules

The subject matter disclosed herein relates to methods of identifying pharmacophores and inhibitors against protein aggregation. The present disclosure also provides pharmacophores themselves and medical uses of agents in the treatment of diseases associated with protein aggregation.

Method of identifying novel protein aggregation inhibitors based on chemical kinetics

Method of tracking a plurality of objects comprising: focusing an image of the objects on an imaging element using an optical system; capturing the image of the objects using an imaging element comprising a plurality of pixels; measuring at least one characteristic of the objects from the captured image using an image processor; wherein the field of view is set be the widest field of view for which the image processor is able to measure the at least one characteristic.

Method of tracking a plurality of objects

Methods for determining the lateral diffusion of one or more components are provided.

Fluidic device

A method for determining the diffusion of one or more components, the method includes the steps of (i) providing a component fluid flow having one or more components; (ii) providing a blank fluid flow; (iii) bringing the flow (i) into contact with the flow (ii) in a large cross section channel, thereby to generate two laminar flows; (iv) permitting the laminar flows generated in (iii) to flow from the large cross section channel into a small cross section channel; measuring the lateral diffusion of the one or more components from the component flow into the blank fluid flow in the small cross section channel. Also provided is a diffusion method having the steps of measuring the lateral diffusion of the one or more components from the component flow into the blank fluid flow at a plurality of diffusion times.

A method for analyzing a component using a fluidic device. The method includes the steps of providing a distribution of the component across contacting first and second fluid flows; diverting a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part includes the component; and analyzing the component in the diverted part of the fluid flow. Optionally the component may be labelled prior to the analyzing step. A flow apparatus for use in the method is also provided.

A prediction method for predicting the effect of an amino acid modification on the rate of aggregation (solubility) of a reference polypeptide comprising: calculating the difference in hydrophobicity (&#x394;Hydr) between the reference polypeptide and a modified polypeptide, calculating the difference in &#x3b2;-sheet propensity (&#x394;&#x394;G+&#x394;&#x394;G) between the reference polypeptide and modified polypeptide, calculating the difference in charge (&#x394; Charge) between the reference polypeptide and modified polypeptide, and calculating: [x* &#x394;Hydr]+[y*(&#x394;&#x394;G+&#x394;&#x394;G)]&#x2212;[z*&#x394; Charge], wherein x, y and z are scaling factors.

Computational method and apparatus for predicting polypeptide aggregation or solubility

A method of determining aggregation rate data predicting an aggregation rate of a polypeptide defined by an amino acid sequence, the method comprising determining a hydrophobicity value, a charge value, and at least one shape propensity value for said sequence; identifying one or more aggregation-influencing patterns within said sequence; determining a pattern value for the sequence responsive to said identifying; and determining said aggregation rate data by determining a weighted combination of said hydrophobicity value, said charge value, said at least one shape propensity value, said pattern value and at least one factor extrinsic to said amino acid sequence.

Method and apparatus for assessing polypeptide aggregation

A prediction method for predicting the effect of an amino acid modification on the rate of aggregation (solubility) of a reference polypeptide comprising: calculating the difference in hydrophobicity (&#x394;Hydr) between the reference polypeptide and a modified polypeptide, calculating the difference in &#x3b2;-sheet propensity (&#x394;&#x394;G+&#x394;&#x394;G) between the reference polypeptide and modified polypeptide, calculating the difference in charge (&#x394; Charge) between the reference polypeptide and modified polypeptide, and calculating: [x*&#x394;Hydr]+[y*(&#x394;&#x394;G+&#x394;&#x394;G)]&#x2212;[z*&#x394;Charge], wherein x, y and z are scaling factors.

A prediction method for predicting the effect of an amino acid modification on the rate of aggregation (solubility) of a reference polypeptide comprising: calculating the difference in hydrophobicity (&#x394;Hydr) between the reference polypeptide and a modified polypeptide, calculating the difference in &#x3b2;-sheet propensity (&#x394;&#x394;G+&#x394;&#x394;G) between the reference polypeptide and modified polypeptide, calculating the difference in charge (&#x394; Charge) between the reference polypeptide and modified polypeptide, and calculating: [x*&#x394;Hydr]+[y*(&#x394;&#x394;G+&#x394;&#x394;G)]&#x2212;[z*&#x394; Charge], wherein x, y and z are scaling factors.

Growing community of inventors

Cambridge, United Kingdom

Christopher Martin Dobson