Li-cor, Inc.

I-cor, Inc.

Christopher Lesiak

Ahmed Bouzid

David L Franzen

Andrew George Ragatz

Sean B Pieper

Mark Fiedler

Irma Nicholls

Filter wheel assemblies with a single actuation point to control positioning of front and rear optical filter elements simultaneously and to provide high channel density with a plurality of selectable optical filter pairs. A filter wheel assembly may include a plurality of optical filter element pairs arranged around a common axis, wherein each of the plurality of optical filter element pairs includes a first filter element and a complementary filter element, wherein each first filter element and each complementary filter element has a surface having a normal component directed toward an inner portion of the filter wheel assembly.

Compact high density rotary optical filter wheel assemblies

Systems and methods are described for extending the dynamic range of imaging systems, and more particularly fluorescence or luminescence imaging systems, having low optical background and a linear detector response. Images of a sample at each of a set of exposure times are acquired, a system-level dark estimate for each exposure time is subtracted from each image to form dark-corrected images, and the different exposures (dark-corrected images) are merged into a wider dynamic-range image. Typically merging is performed on a pixel-by pixel basis.

Wide dynamic range imaging

Systems and methods for flattening the image across the entire field by correcting the image for both the fluorescence and scatter spatial variations. Images of a stable fluorescence target and a scattering target are separately acquired in an imaging system. From these target images, a pixel remapping function, e.g., including two correcting pixel slopes, is calculated for subsequent image pixel remapping. An image of a sample under investigation is then acquired by the imaging system and the sample image is remapped based on the pixel remapping function for the imaging system to form a corrected (field flattened) image. Which correction pixel slope to be used is determined based on whether a sample image pixel value is higher or lower than a threshold value.

Field flattening correction method for fluorescence imaging system

A multi-channel scanning system adapted to implement a low channel cross-talk, extra-wide dynamic range scanning method by scanning the same location more than once, wherein at least one time, the power of the excitation light and detector gain are set to High for at least one of the channels and Low for at least one of the other channels and different settings are used in subsequent scans. The scans of the same channel taken with different High and Low settings are merged together to produce one wider dynamic range image.

High dynamic range scanning with reduced channel cross-talk

A spectral filtering apparatus and method is presented, which enables the functions of hyperspectral imaging to be performed with increased speed, simplicity, and performance that are required for commercial products. The apparatus includes a photosensitive array having a plurality of photosensitive elements such that different subsections of photosensitive elements of the array receive light of a different wavelength range of a characteristic spectrum of a target, and output electronics combines signals of at least two photosensitive elements in a subsection of the array and outputs the combined signal as a measure of the optical energy within a bandwidth of interest of the characteristic spectrum.

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Lincoln, NE, United States of America

Christopher Lesiak