Harvard College

Blueprint Medicines Corporation

Christopher Lee

Andrew Levin

Caitlin N Kinkema

Erika Butler

A polypeptide is located in a gel matrix by treating the matrix with a negative staining agent (i.e. an agent that stains the gel more intensely in regions devoid of polypeptides, so that gel areas exhibiting reduced staining intensity are indicative of the presence of polypeptide). Specifically, the staining agent comprises a copper or cobalt salt, and a detergent. The term polypeptide is used to include polypeptides of all sizes, including proteins and glycoproteins. The polypeptide can be purified by applying it to a gel matrix, causing it to run into the matrix, applying the above-described staining agent and then eluting the polypeptides from the matrix.

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Cambridge, MA, United States of America

Christopher Lee