Glykos Finland Oy

Vtt Technical Research Centre of Finland Ltd

Christopher Landowski

Markku Saloheimo

Anne Kanerva

Ann Westerholm-Parvinen

Anne Huuskonen

Jari Natunen

Juhani Saarinen

Anna-Liisa Hänninen

Noora Salovuori

Jukka Hiltunen

Merja Elisa Penttila

Merja Penttilä

Annamari Heiskanen

Bernhard Helk

Hanna Salo

Heli Viskari

Christian Ostermeier

Benjamin Patrick Sommer

Ramon Wahl

Heidi Salminen

Georg Schmidt

Eero Mustalahti

Jussi Joensuu

Anne Leppänen

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Protease deficient filamentous fungal cells and methods of use thereof

Multiple proteases deficient filamentous fungal cells and methods of use thereof

Multiple protease deficient filamentous fungal cells and methods of use thereof

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

Described herein are compositions including filamentous fungal cells, such asfungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example,fungal cells, as the expression system.

Production of fucosylated glycoproteins

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Helsinki, Finland

Christopher Landowski