Zymogenetics, Inc.

Resolution Bioscience, Inc.

Other

Merck Sharp + Dohme Corp.

Life Technologies Corporation

Rosetta Inpharmatics, Inc.

Salish Bioscience Inc.

Christopher K Raymond

Christopher D Armour

Erica Vanaja

Michael R Stamm

Paul D Bishop

Thomas R Bukowski

Susan D Holderman

Philip W Garrett-Engele

Jennifer Hernandez

Zhengyan Kan

John C Castle

James S Sloan

Brady G Miller

The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provides methods for the sensitive and specific detection of target gene sequences and gene expression profiles.

Methods for quantitative genetic analysis of cell free DNA

The disclosure provides methods and reagents for preparing DNA libraries from biological materials for targeted sequencing. The approach can enhance the efficiency and sensitivity of targeted sequencing applications, such as liquid biopsy analyses to assess genetically driven conditions. In an embodiment, the disclosed method comprises attaching the 5' end of an oligonucleotide adapter to the 3′ end of double-stranded DNA fragment to produce an adapter/fragment chimeric molecule; producing at least one complementary strand of the adapter/fragment chimeric molecule by linear amplification; hybridizing the at least one complementary strand with an oligonucleotide probe, wherein the oligonucleotide probe comprises a hybridization domain with a sequence that hybridizes to a target sequence in the complement strand to produce a targeted complement strand/probe duplex; purifying the targeted complement strand/probe duplex; and extending the probe in the purified targeted complement strand/probe duplex and amplifying with PCR to produce a plurality of sequencing template molecules.

Methods and composition for targeted genomic analysis

The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provide methods for the sensitive and specific detection of target gene sequences and gene expression profiles.

Methods for targeted genomic analysis

The invention provides a method for efficient DNA library construction and targeted genetic analyses of the libraries.

High efficiency construction of DNA libraries

The present invention includes compositions and methods useful for the detection of a mutational change, SNP, translocation, inversion, deletion, change in copy number, or other genetic variation within a sample of cellular genomic DNA or cell-free DNA (cfDNA). In some embodiments, the compositions and methods of the present invention provide an extremely high level of resolution that is particularly useful in detecting copy number variations in a small fraction of the total cfDNA from a biological sample (e.g., blood).

Methods for the detection of genomic copy changes in DNA samples

Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5' region having a first restriction enzyme recognition sequence and flanked by a 3&#x2032; region having a second restriction enzyme recognition sequence, and a region which hybridizes to a capture nucleic acid molecule. The single-stranded nucleic acid probes are useful for solution-based capture methods.

Methods for generating target specific probes for solution based capture

In one aspect, the present invention provides methods for amplifying a microRNA molecule to produce DNA molecules. The methods each include the steps of: (a) using primer extension to make a DNA molecule that is complementary to a target microRNA molecule; and (b) using a universal forward primer and a reverse primer to amplify the DNA molecule to produce amplified DNA molecules. In some embodiments of the method, at least one of the forward primer and the reverse primer comprise at least one locked nucleic acid molecule.

Methods for quantitating small RNA molecules

Novelsecretory signal polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods of using are disclosed. Methods of producing large amounts of recombinant proteins by employing DNA constructs having a polypeptide of interest preceded by a novelsecretory signal sequence.

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The present invention features nucleic acids and polypeptides encoding novel splice variant isoforms of acetyl-CoA carboxylase 2 (ACC2). The polynucleotide sequence of ACC2sv1 is provided by SEQ ID NO 3. The amino acid sequence of ACC2sv1 is provided by SEQ ID NO 4. The present invention also provides methods for using ACC2sv1 polynucleotides and proteins to screen for compounds that bind to ACC2sv1.

Alternatively spliced isoform of acetyl-CoA carboxylase 2 (ACC2)

Pichia methanolica secretory signal

Transcription promoter and terminator sequences from the glyceraldehyde-3-phosphate dehydrogenase 1 gene (GAP1 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured cells. Within the expression vectors, a GAP1 promoter and/or a GAP1 terminator is operably linked to a DNA segment encoding the protein of interest.

Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 1 promoter and terminator

Transcription promoter and terminator sequences from the glyceraldehyde-3-phosphate dehydrogenase 2 gene (GAP2 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured cells. Within the expression vectors, a GAP2 promoter and/or a GAP2 terminator is operably linked to a DNA segment encoding the protein of interest.

Growing community of inventors

Seattle, WA, United States of America

Christopher K Raymond