Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Corteva Agriscience LLC

Christopher Jay Scelonge

Dennis L Bidney

William James Gordon-Kamm

Keith S Lowe

Igor Falak

Joshua K Young

David George Charne

Glenn S Cole

Cathy Xiaoyan Zhong

James C Register, Iii

Mark K Mancl

M Alejandra Pascual

Rebecca E Cahoon

Saverio Carl Falco

Zhongsen Li

Yumin Tao

Katherine A P Nazarian

Albert Laurence Lu

Michael W Lassner

Scott H Diehn

David J Peterson

Timothy M Nowatzki

Erin L Crowgey

Douglas S Nubel

Andrew Mark Cigan

Kevin E McBride

Theodore Mitchell Klein

Huirong Gao

Ning Wang

Lijuan Wang

Bing-Bing Wang

Zhan-Bin Liu

Sean J Coughlan

Craig E Hastings

Matthew Curtis Harmon

Grace Marie St Clair

Dean Podlich

Clara Maria Alarcon

Jasdeep Mutti

Carl R Simmons

Benjamin A Bowen

Guihua Lu

Gary A Huffman

Michael D Miller

Wesley B Bruce

Leszek Aleksander Lyznik

Gary A Sandahl

Zhan-bin Liu

Dorothy A Pierce

Xinli Emily Wu

Margit C Ross

Douglas Stuart Nubel

Lisa Newman

Katherine A Nazarian

Aurélie Morin

Lawrence R Stiner

William Gordan-Kamm

Andre A Steinmetz

Rachel Yvonne Baltz

Grace M St Clair

Michael Lassnser

Kevin E Mcbride

James Calvin Register

M Alejandra Villanelo

Mike Miller

Gary Sandahl

Clara M Alarcon

James C Iii Register

Maria Alejandra Pascual

Iii James Calvin Register

M Alejandra Pascual Villanelo

James C Register

Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

Generation of site specific integration sites for complex trait loci in corn and soybean, and methods of use

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for generating complex trait loci

Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use

Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Methods and compositions for the introduction and regulated expression of genes in plants

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Maize event DP-004114-3 and methods for detection thereof

A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Methods and compositions for targeted polynucleotide modification

Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for targeted integration in a plant

A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excize a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Growing community of inventors

Ankeny, IA, United States of America

Christopher Jay Scelonge