Zymogenetics, Inc.

Christopher J Stenland

Paul O Sheppard

Paul D Bishop

John W Forstrom

Tracey A Pownder

Karen S De Jongh

Robert W Mallett

Jonathon C Boone

Robert W Mallet

Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.

Thrombin activator compositions and methods of making and using the same

Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

Method for activating prethrombin-1

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Stanwood, WA, United States of America

Christopher J Stenland