Syngenta Participations Ag

Christopher J Batie

Michael B Lanahan

Shib Sankar Basu

Joyce Craig

Wen Chen

George W Aux

Mark Kinkema

Edward S Cates

Jay Austin Dinwiddie

Gregg Crabb

Carl A Miller

Aron Silverstone

Rene Quadt

Mark D Kinkema

Greg Crabb

The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are 'self-processing' transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.

Self-processing plants and plant parts

The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.

Process for starch liquefaction and fermentation

Self processing plants and plant parts

Growing community of inventors

Durham, NC, United States of America

Christopher J Batie