Genzyme Corporation

Christopher Hwang

Cheng Cheng

Jonathan Wang

Jason Walther

Neha Shah

Seul-A Bae

Konstantin Konstantinov

Timothy Allan Johnson

Veena Warikoo

Christine T DeMaria

Rao Koduri

Marcella Yu

Jin Yin

Timothy Johnson

Karen Lee

Kevin P Brower

Christine Demaria

Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Methods of culturing a mammalian cell

Provided herein are recombinant glycoproteins (e.g., recombinant human &#x3b1;-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human &#x3b1;-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human &#x3b1;-galactosidase-A protein).

Recombinant glycoproteins and uses thereof

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Cambridge, MA, United States of America

Christopher Hwang