10x Genomics, Inc.

Bio-rad Laboratories, Inc.

Christopher Hindson

Serge Saxonov

Michael Schnall-Levin

Benjamin J Hindson

Kevin D Ness

Mirna Jarosz

Andrew D Price

Paul Hardenbol

Rajiv Bharadwaj

Donald Masquelier

Anthony Makarewicz

Katherine Pfeiffer

Phillip Belgrader

Mohammad Rahimi Lenji

John R Stuelpnagel

Debkishore Mitra

Tobias Daniel Wheeler

Adam Lowe

Xinying Zheng

Tarjei Sigurd Mikkelsen

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Josephine Harada

Elliott Meer

Yuan Li

Christopher Joachim O'Keeffe

Pranav Patel

Bill Kengli Lin

Yifeng Yin

Shalini Gohil

Joshua Delaney

Solongo B Ziraldo

Geoffrey McDermott

Zahra Kamila Belhocine

Vijay Kumar Sreenivasa Gopalan

Joseph Francis Shuga

Michael Ybarra Lucero

Amy L Hiddessen

Chunxiao Han

Erin Rae Chia

Niranjan Srinivas

Indira Wu

Stephane Claude Boutet

Sarah Taylor

Geoff McDermott

Yaqi Wang

Patrick Marks

Kamila Belhocine

Mima Jarosz

Landon Merrill

The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

Analysis of nucleic acid sequences

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

Systems and methods for sample analysis

Methods and systems for cell and bead processing or analysis are provided. A cell or bead can be subjected to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). The cell or bead can further be subjected to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks are formed within the cell or bead.

Methods and systems for cell and bead processing

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same

The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

Compositions, methods, and systems for bead formation using improved polymers

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides methods and systems for cell and bead processing or analysis. A method for processing a cell or bead may include subjecting a bead to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). Such a method may further include subjecting the cell or bead to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks may be formed within the cell or bead.

Compositions and methods for sample processing

Growing community of inventors

Pleasanton, CA, United States of America

Christopher Hindson