Iogen Energy Corporation

Codexis, Inc.

Novozymes A/s

Christopher Hill

John J Tomashek

Brian R Scott

Chengsong Liu

Andrew Shaw

Ish Kumar Dhawan

Dipnath Baidyaroy

James A Lavigne

Loreta Gudynaite-Savitch

Oleh Tanchak

Amala Chokshi

Nabil Masri

Christopher D Hindle

Martine Whissel

Guylaine Therrien

Brian Scott

Sophie Calixte

Fuad Tahna

Jacqueline Montalibet

Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.

Cellobiohydrolase enzymes

The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and Family 5 cellulases or the Family 45 and Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing the foregoing cellulases. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.

Cellulase enzyme mixtures for depilling and uses thereof

The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.

Compositions and methods for production of fermentable sugars

Provided are modified beta-glucosidase enzymes, derived from theCel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.

Modified beta-glucosidases with improved stability

Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (f) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (f) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (f) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes

An enzyme mixture for the enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars is provided. Also provided is a process for hydrolyzing a pretreated lignocellulosic feedstock with an enzyme mixture. The enzymatic hydrolysis comprises adding a cellulase enzyme mixture to a pretreated lignocellulosic feedstock. The cellulase enzyme mixture comprises a primary cellulase mixture of CBH1 and CBH2, and EG1 and EG2. The CBH1 and CBH2 being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture. Furthermore, CBH2 is present at a fraction relative to CBH1 and CBH2, and EG2 is present at a fraction relative to the EG1 and EG2.

Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks

Growing community of inventors

Nepean, Canada

Christopher Hill