Johns Hopkins University

Christopher Hadiono

Hongjun Song

Guoli Ming

Ha Nam Nguyen

Mingxi Max Song

Xuyu Qian

William Jeang

A method of cell culture and a method of generating organoids is provided. The methods utilize a cell culture system which includes a multiwell culture plate having shafts for mixing culture media within wells of the culture plate. The multiwell culture plate includes a base substrate having a plurality of culture wells, and a shaft operably associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well. The system further includes a motor having a drive shaft in operable communication with the shaft gears of the multiwell culture plate. The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

Multiwell cell culture system having rotating shafts for mixing culture media and method of use thereof

A cell culture system and method of generating organoids is provided. The system includes a multiwell culture plate having shafts for mixing culture media within wells of the culture plate. The multiwell culture plate includes a base substrate having a plurality of culture wells, and a shaft operably associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well. The system further includes a motor having a drive shaft in operable communication with the shaft gears of the multiwell culture plate. The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

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Armonk, NY, United States of America

Christopher Hadiono