USA As Represented by Secretary of the Navy

Lumacyte, LLC

Lumactye, Inc.

Theia Scientific, LLC

Christopher Field

Sean J Hart

Colin Hebert

Shweta Krishnan

Pehr E Pehrsson

Junghoon Yeom

Kevin J Johnson

Braden C Giordano

Daniel Ratchford

Alexander V Terray

Michael Paul Malito

Hyun Jin In

Adam L Lubrano

Zachary Evans

Adam Lubrano

Nathan LaPuma

Duane A Rogers

Kevin Field

Margaret McCoy

A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

Apparatus for holding a microfluidic chip

Provided are devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises automated flow, wherein the samples comprise liquid or particles in a sample vessel, and wherein the devices comprise an assembly of components that enable processing of a sample for analytical assessment by fluidic and/or particle based instruments. Automated flow may comprise systems for moving samples including vacuum systems, pressure-based systems, pneumatic systems, pumps, peristaltic pumps, diaphragms, or syringes. The devices may comprise an assembly of components that enable movement in X, Y, and Z dimensions, as well as switches, microfluidic tubing, well plate block, electronic pressure controllers, pneumatic or fluidic mixing devices, components for fluid handling, sampling vessels, and mechanical components for translating or transporting system components.

Fluidic autosampler and incubator

A system and method for processing multi-modal microscopy imaging data on small-scale computer architecture which avoids restrictive manufacturer data formats and APIs. The system and method leverage a web-based application made available to microscopy instrument control hardware by which direct visual output of the control hardware is captured and transmitted to an edge computing device for processing by one or more inference models in parallel to construct a composite hyperimage.

System and method for multi-modal microscopy

Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

Disclosed herein is a method of providing a structure having two electrodes connected by nanowires, exposing the structure to an analyte that can adsorb onto the nanowires, and passing an electrical current through the nanowires to heat the nanowires to desorb the analyte. Also disclosed herein is an apparatus having the above structure; a current source electrically connected to the electrodes, and a detector to detect the analyte.

Nanowire arrays for trace vapor preconcentration

Disclosed herein is a structure having: a support, a plurality of nanowires perpendicular to the support, and an electrode in contact with a first end of each nanowire. Each nanowire has a second end in contact with the support. The electrode contains a plurality of perforations. The electrode contains a plurality of perforations. Also disclosed herein is a method of: providing the above support and nanowires; depositing a layer of a filler material that covers a portion of each nanowire and leaves a first end of each nanowire exposed; depositing a plurality of nanoparticles onto the filler material; depositing an electrode material on the nanoparticles, the ends of the nanowires, and any exposed filler material; and removing the nanoparticles and filler material to form an electrode in contact with the first end of each nanowire; wherein the electrode contains a plurality of perforations.

Perforated contact electrode on vertical nanowire array

A method and system for generating vapors of liquid solutions where a pneumatically modulated liquid delivery system maintains a defined flow rate for a liquid solution. The pneumatically modulated liquid delivery system includes a flow meter configured to measure a flow rate for the liquid solution flowing from a pressure vessel; a microcontroller configured to determine a pressure necessary to achieve the defined flow rate; and an electronic pressure control unit configured to adjust the pressure inside the pressure vessel. The system includes a nebulizer coupled to the pneumatically modulated liquid delivery system configured to receive the liquid solution from the pneumatically modulated liquid delivery system at the defined flow rate and configured to generate an aerosol of the liquid solution. A heat tube can vaporize the aerosol generated by the nebulizer.

Method and system for generating vapors of liquid solutions

Growing community of inventors

Arlington, VA, United States of America

Christopher Field