Cornell University

Other

Rutgers, the State University of New Jersey

Christopher E Mason

Samie R Jaffrey

Craig S Brown

Kate D Meyer

Delia Tomoiaga

Jeffrey Rosenfeld

Marc T Beck

Jeffrey R Rosenfeld

The present disclosure is directed to methods for typing and characterizing sperm. The technology employs a sequencing-based method for detecting and measuring RNA transcripts from single sperm cells and the analysis of the sequencing data for the prediction of male parent contribution to autism.

Single sperm gene expression and mutation analysis for prediction of diseases

This invention relates to a method of characterizing the modified base status of a transcriptome, which involves contacting a transcriptome comprising one or more modified bases with an antibody specific to the modified bases under conditions effective to bind the antibody to the modified bases; isolating, from the transcriptome, a pool of RNA transcripts to which the antibody binds; and identifying isolated RNA transcripts that are present in a higher abundance in the isolated pool relative to the transcriptome, where each of the isolated RNA transcripts that are present in a higher abundance in the isolated pool together characterize the modified base status of the transcriptome. Also disclosed are a method of diagnosis or prognosis of a disease, a method of determining the effect of a treatment on modified base levels in RNA, and a kit for characterizing the modified base status of a transcriptome.

Methods and kit for characterizing the modified base status of a transcriptome

A message board cache system comprising: a database containing message board information; a notification system in communication with the database, the notification system generating a notification when data in the database is altered or deleted; a web application executing a message board interface, the web application receiving the notification from the notification system and the data altered or deleted; a cache implementation in communication with the web application, the web application deleting an item from the cache implementation upon receiving the notification that the data has been altered or deleted from the database.

Method, system and storage medium for implementing a message board cache system

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Ithaca, NY, United States of America

Christopher E Mason