Emulate, Inc.

Harvard College

Cedars-sinai Medical Center

Vanderbilt University

Janssen Biotech, Inc.

President and Fellows of Havard College

Christopher David Hinojosa

Daniel Levner

Geraldine A Hamilton

Josiah Daniel Sliz

Joshua Gomes

Norman Wen

Donald Elliot Ingber

Guy Robert Thompson, Ii

Jacob Fraser

Jose Fernandez-Alcon

S Jordan Kerns

Antonio Varone

Catherine Karalis

Justin Nguyen

Clive N Svendsen

Dhruv Sareen

Kyung Jin Jang

David Conegliano

Guy Thompson

Carol Lucchesi

Jacob Freake

Doug Sabin

Riccardo Barrile

Carolina Lucchesi

Hyoungshin Park

Jefferson Puerta

Monicah Otieno

Andries Van Der Meer

Kevin Kit Parker

Josue A Goss

Matthew Daniel Solomon

Gad Vatine

Robert Cunningham

Anthony Bahinski

Petrus Wilhelmus Martinus Van Ruijven

Christian Alexander Potzner

Patrick Sean Tuohy

Magdalena Kasendra

Remi Villenave

Samuel Sances

Michael Workman

Stephen R Targan

Uthra Rajamani

Grace Ahn

Kambez Hajipouran Benam

Lina Williamson

Hubert Geisler

Bryan Hassell

Sonalee Barthakur

Tanvi Shroff

Brian Zuckerman

Karel Domansky

Victor Antontsev

Jonathan Rubins

Iosif Pediaditakis

Guy Thompson, Iii

Donald E Ingber

Guy Robert Thompson

Hyoung Shin Park

Josiah Sliz

John P Wikswo

Ronald S Reiserer

Dmitry A Markov

David Edward Cliffel

Philip C Samson

Richard Novak

Lisa J McCawley

Craig Henshaw

Lewis Rowe

Gadi Pelled

Dan Gazit

Frank Emmanuel Block

Zulma Gazit

Georg Bauer

Dmitriy Sheyn

John A Mclean

Alfred Paris

Jennifer Robin McKenzie

Abhishek Jain

Ville Kujala

Andries D Van Der Meer

Daniel Patterson

SJordan Kerns

Marinke Van Der Helm

Marjon Zamani

Samuel Sanees

David James Coon

Josh Gomes

Thomas Umundum

Anjoscha Kaus

Berhan Mandefro

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Removing bubbles in a microfluidic device

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

Microfluidic model of the blood brain barrier

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Compound distribution in microfluidic devices

Development of spinal cord on a microfluidic chip

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

Integrated human organ-on-chip microphysiological systems

An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Organomimetic devices and methods of use and manufacturing thereof

The invention generally relates to a microfluidic platforms or 'chips' for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

Effects of space travel on human brain cells

Microfluidic 'organ-on-a-chip' devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

Method of osteogenic differentiation in microfluidic tissue culture systems

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

Systems and methods for cell culture device interconnection and fluidic device interconnection

The present invention is related to the field of fluidic devices, and in particular, microfluidic cell culture systems. The present invention provides pumping, recirculation and sampling for a microfluidic device or devices. The present invention provides solutions to the control of microfluidics for both terrestrial and space applications, including the control over the movement of fluids in zero gravity or microgravity.

Microfluidic control

Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

Low shear microfluidic devices and methods of use and manufacturing thereof

Growing community of inventors

Cambridge, MA, United States of America

Christopher David Hinojosa