Wake Forest University

Christopher Daniel Porada

Aleksander Skardal

Maria Graca Almeida-Porada

Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or 'niches') selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2&#x2212;; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.

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Winston-Salem, NC, United States of America

Christopher Daniel Porada