Dartmouth College

Enchi Corporation

Ut-battelle, Inc.

Mascoma Corporation

Christopher D Herring

Nicky Caiazza

Arthur J Shaw, Iv

Johannes Pieter Van Dijken

Daniel G Olson

Aaron Argyros

Trisha Barrett

Adam Michael Guss

John S Bardsley

Frank Agbogbo

D Aaron Argyros

Yu Deng

Stephen R Rogers

Chaogang Liu

Jonathan Lo

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Engineering an increase in ethanol production by altering cofactor specificity

The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Engineering microorganisms to increase ethanol production by metabolic redirection

The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

Flow-through biological conversion of lignocellulosic biomass

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Lebanon, NH, United States of America

Christopher D Herring