Fluent Biosciences Inc.

Christopher D'amato

Sepehr Kiani

Robert Meltzer

Yi Xue

Kristina Fontanez

This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.

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Wellesley, MA, United States of America

Christopher D'amato