Bio-rad Laboratories, Inc.

Christopher Belisle

Jiali Liao

Hong Chen

Xuemei He

Yueping Xu

Lee Olech

Mark A Snyder

Thomas R Berkelman

Xuemei Yang

Lei Li

Praveena D Garimella

Edwin Lozano

William Strong

Clayton T McKee

James W Xu

Haihui Yuan

Leila Amery Ranis

Fang Song

Xiaozhong Bao

Juan You

Wenli Cao

Clayton T Mckee

Garimella Praveena

Methods of preparing polymer-filled chromatography resin and their uses are provided.

Method of preparing polymer-filled chromatography resin

Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.

Anionic exchange-hydrophobic mixed mode chromatography resins

Chromatography resins having anionic exchange-hydrophobic mixed mode ligands, that are useful for purifying target biomolecules using anionic exchange (i.e., where the ligand is positively charged) and hydrophobic mixed mode chromatography. The chromatography resins allow for efficient purification of target biomolecules (e.g., recombinant proteins, antibodies, antibody-drug conjugates, or antibody derivatives including, but not limited to, antibody fragments and antibody fusions) from a sample, and have been found to be useful in purifying monomeric target biomolecules from aggregate target biomolecules. In an embodiment, the chromatography resins are useful for separating antibodies from one or more components (e.g., contaminants) in the sample.

Anionic exchange-hydrophobic mixed mode chromatography resin

A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.

Dynamic axial compression for preparative columns using external compression

Polymer dots comprising a functionalized fluorescent polymer and an amphiphilic molecule are provided.

Polymer dots

The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-dioxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.

Mixed mode cation exchange chromatography ligands based on 1,3-dioxoisoindolin-2-yl structures

Chromatography resins having mixed mode ligands and methods of using such resins are provided.

Chromatography resin having an anionic exchange-hydrophobic mixed mode ligand

Polymer-filled ceramic apatites and their uses are provided.

Agarose-filled ceramic apatite

Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.

Modified electrode buffers for stain-free protein detection in electrophoresis

Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.

Growing community of inventors

Walnut Creek, CA, United States of America

Christopher Belisle