Cargill Inc.

Christopher Austin Tyler

Troy Allen Rhonemus

Andrew Keith Ohmes

Allan S Myerson

John J Hahn

Jeffrey C Evans

Aron Broman Erickson

Kern M Storo

Ting Liu Carlson

John Joseph Hahn

Aron Erickson

Ronny Leontina Marcel Vercauteren

Robert J Brower, Iii

An Amanda Jules Heylen

Alexander Patist

Jenna Stangler

Timothy Oolman

Bruce Michael Furlano

Jenna Helgeson

Tougeu Vang

Chen-Chou Chiang

Robert Joshua Brower Iii

Alan Stuart Myerson

The invention relates to the use of adsorb/desorb chromatography to prepare enriched compositions comprising rebaudioside B and/or rebaudioside D. Compositions with enriched rebaudioside-B and/or rebaudioside-D components may be prepared from-derived glycoside compositions using an adsorb-desorb chromatography process where the stationary phase of the chromatography bed comprises a macroporous neutral adsorbent resin.

Method for the enrichment of rebaudioside b and/or rebaudioside d in stevia-derived glycoside compositions using adsorb-desorb chromatography with a macroporous neutral adsorbent resin

A process for production of crystalline rebaudioside B from rebaudioside A is described. Additionally, four distinct crystal polymorphic forms of rebaudioside B are described, as well as methods for converting from one crystal polymorphic form of rebaudioside B to another crystal polymorphic form of rebaudioside B.

Crystalline forms of rebaudioside B

The present invention provides methods to treat mixtures containing natural rebaudioside A (Reb A), rebaudioside B (Reb B), and rebaudioside D (Reb D), synthetic counterparts of these, and/or derivatives of the natural or synthetic embodiments obtain one or more of these glycosides in more pure form. In many embodiments, the invention can be used to process glycoside mixtures obtained at least in part from natural sources such as theplant. This allows, for instance, the recovery of a product including Reb A material in more pure form relative to Reb B material or Reb D material. As an alternative or in addition to recovery of the purified Reb A material, a product including Reb B material and/or Reb D material in more pure form relative to Reb A material can be obtained.

Methods to treat mixtures of glycosides to obtain one or more of these glycosides in more pure form

Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

Separation of rebaudioside A from stevia glycosides using chromatography

The invention provides methods of purifying rebaudioside A from a mixture comprising glycosides of the plant. The methods of the invention are useful for preparing highly pure rebaudioside A compositions from crudestarting compositions that are typically considerably lower in rebaudioside A concentration. The highly pure rebaudioside A compositions are useful as non-caloric sweeteners in edible or chewable compositions such as food, beverages, medicine, candy, chewing gum, and the like.

Method of producing purified rebaudioside A compositions using solvent/antisolvent crystallization

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Minnetonka, MN, United States of America

Christopher Austin Tyler