Massachusetts Institute of Technology

University of California

Harvard College

The Broad Institute, Inc.

Christopher A Voigt

Dehua Zhao

Karsten Temme

Ethan Mirsky

Travis Scott Bayer

Michael Joseph Smanski

Fang-Yuan Chang

Min-Hyung Ryu

Angela M Belcher

Timothy Kuan-Ta Lu

Wendell A Lim

Robert Nicol

Jon Clardy

Michael A Fischbach

Anselm Levskaya

Tarjei Mikkelsen

Thomas Wyche

Uyanga Tsedev

Virgil Rhodius

Mark Kyle Mimee

Andrea Kimi Wallace

Maiko Furubayashi

Chun-Jun Guo

Carol A Gross

Johann Elbaz

Mao Taketani

Mi Ryoung Song

Alex C Tucker

Di Sheng Lee

Tae-Gon Cha

Lauren Andrews

A colored composition having a self-assembly of a plurality of a peptide and melanin, and methods of producing and using the colored composition are described.

Melanin-peptide-based photonic materials

Disclosed herein are engineered rhizobia having nif clusters that enable the fixation of nitrogen under free-living conditions, as well as ammonium and oxygen tolerant nitrogen fixation under free-living conditions. Also provided are methods for producing nitrogen for consumption by a cereal crop using these engineered rhizobia.

Control of nitrogen fixation in rhizobia that associate with cereals

Methods for making synthetic gene clusters are described.

Synthetic gene clusters

Provided herein, in some aspects, are methods and compositions for producing single-stranded DNA (ssDNA) having uniform length.

Optimization of circular single stranded DNA using M13 phage

The invention relates to methods and products for generating diverse libraries of genetic material. The products include libraries and constructed nucleic acids as well as kits and databases and systems thereof.

Directed evolution of synthetic gene cluster

Disclosed is a method of generating a set of sequence-verified nucleic acid elements for the combinatorial construction of genetic elements. The method includes: providing a plurality of nucleic acid parts; assembling nucleic acid parts to form a one or more nucleic acid elements, wherein the nucleic acid elements include at least two sequences selected from the plurality of parts; and determining the sequence of the nucleic acid elements. Further disclosed is a pool of higher-order nucleic acid constructs or amplification products thereof, comprising one or more nucleic acid elements as well as kits including a pool of sequence-verified nucleic acid elements of claims and/or a pool of higher-order nucleic acid constructs; and a plurality of primers for retrieving one or more sequence-verified nucleic acid elements and/or higher-order nucleic acid constructs.

High-throughput assembly of genetic elements

Provided herein, in some aspects, are tools (e.g., methods, compositions and nucleic acids) for building genetic circuits inandbacteria, as well as the bacteria containing the genetic circuits.

Gene expression in Bacteroides

The invention relates to methods for promoting fixed nitrogen from atmospheric nitrogen, and related products. Endophytic bacteria having an exogenous nif cluster promote fixed nitrogen for cereal plants.

Nitrogen fixation using refactored NIF clusters

Disclosed herein, in some embodiments, are vectors encoding biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes), engineered cells comprising the vectors, and methods of using biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes) to produce fatty acid amides.

Human gut microbiome-derived biosynthetic enzymes for production of fatty acid amides

The present disclosure relates, in some aspects, to bile acid sensors.

Transcriptional sensor for bile acids in bacteroides thetaiotaomicron

Disclosed herein, inter alia, are compositions for modulating protease activity and treating cancer.

Compositions and methods for modulating protease activity

Described herein are novel synthetic molecules, polymers, and compositions comprising silica-binding peptides, and their methods of production. Also described herein are methods of synthesizing structurally and chemically complex silica-based materials using the synthetic molecules, polymers, and compositions.

Peptide-based synthetic molecules and silica nanostructures

The invention relates to anti-sigma factors ('anti-sigmas') that bind to sigma factors and block activation of transcription. Anti-sigmas and their cognate sigma factors provide a highly effective mechanism for regulating gene expression in genetic circuits.

Growing community of inventors

Oakland, CA, United States of America

Christopher A Voigt