Institut National De La Sante Et De La Recherche Medicale

Other

Aventis Pharma S.a.

Genopoietic

Christophe Torrent

Jean-Luc Darlix

Michel Perricaudet

Patrice Yeh

David Klatzmann

Jean-Loup Salzmann

Sandrine Jacquemoud

Clarisse Berlioz

The invention concerns methods and constructs for producing retroviral particles, in vitro, ex vivo or in vivo. It also concerns the use of said methods and constructs for transferring nucleic acids into cells. More particularly, the invention concerns a composition comprising the whole set of genetic elements required for constituting a retroviral particle, incorporated in one or several recombinant adenoviruses defective for all or part of the regions E1 and E4 at least (adenoviral/retroviral chimeric vectors).

Methods and compositions for producing viral particles

Internal ribosome entry site, vector containing it and therapeutic use

The invention proposes a packaging signal (psi) region of at most 400 nucleotides, capable of promoting encapsidation of a retroviral vector, which is substantially homologous to the 5' end of a murine viral-like cellular sequence of type VL30, as well as a retroviral vector comprising said psi region.

Retroviral vectors comprising a VL30-derived psi region

Growing community of inventors

Paris, France

Christophe Torrent