Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur

Christophe Pannetier

Philippe Kourilsky

Madeleine Cochet

Sylvie Darche

A method for determining the nucleotide size of DNA fragments separated by gel electrophoresis, comprising the steps of (i) measuring the migration time of each detected DNA fragment, and (ii) correlating the size of each detected DNA fragment with its migration time.

Method for determining the nucleotide size of DNA fragments

The present invention relates to a process for determining the quantity of a DNA fragment of interest in a sample wherein:

Process for determining the quantity of a DNA fragment of interest by a

In a method for describing repertoires of antibodies (Ab) and T cell recers (TcR) of the immune system of an individual, reverse transcription is carried out on the mRNA contained in a biological sample, separate amplifications are then carried out on the transcription product (or directly on the DNA extracted from the sample) by a PCR type method for each primer pair V, C, where V corresponds to a variable segment of the repertoire of interest and C hybridizes on the constant segment of the repertoire of interest. For each J segment of the labelled repertoire, an elongation step is carried out on each of the amplification products using a specific oligonucleotide of this segment J as primer and the amplification product as matrix. For each elongation product corresponding to a triplet (V, C)J thus obtained, the size of the different elongation products is revealed. The description of the repertoires corresponds to a VCJ triplet and to the element size for each element of the repertoire.

Growing community of inventors

Paris, France

Christophe Pannetier