Dupont Nutrition Biosciences Aps

International N&h Denmark Aps

Rhodia Chimie

Universite Laval (canada)

Danisco A/s

Institut National De La Recherche Agronomique (inra)

Texel LLC

Danisco France

Christophe Fremaux

Philippe Horvath

Dennis A Romero

Patrick Boyaval

Rodolphe Barrangou

Pascal Fourcassie

Elise Manoury

Pascal Hols

Yves Cenatiempo

Sylvain Moineau

Laetitia Fontaine

Laurence Simon

Alexander Hynes

Geneviève Rousseau

Jean-Marie Berjeaud

Thomas Desfougeres

Joshua K Young

Simon Labrie

Dennis Romero

Anaïs Jedrzejowski

Jerome Mengaud

Sonia Huppert

Juliette Hagege

Sabine Van Dillen

Pierre Renault

Max Charles Jodeau

Iris Besancon-Yoshpe

Armelle Cochu-blachère

Armelle Cochu-Blachére

Frederic Toussaint

Stephane Chaillou

Thomas Desfougéres

Joachim Schwobe

Damien Lugand

Franck Biet

Phillippe Horvath

Armelle Cochu-Blachere

Jamila Anba

Marie-Laurence Lemay

David Blandine

Amandine Radziejwoski

Jean-Marc Berjeaud

Frédéric Toussaint

The invention relates to a polynucleotide comprising a lacZ gene (lacZ) encoding a β-galactosidase characterized by a particular profile regarding its efficiency of hydrolysis of lactose. The invention is also directed to astrain comprising a lacZallele and bacterial composition thereof, and their use to obtain fermented milk not undergoing post-acidification.

Lactic acid bacteria

The present invention relates to a method for transforming a strain of thegenus through natural competence. The present invention further relates to strains obtained or obtainable by said method. The present invention also relates to a method for identifying a strain of thegenus which is transformable through natural competence.

Methods and strain

The invention relates to a lactose-positive, galactose-negative,strain carrying a mutation in 2 or 3 genes selected from the group consisting of 1) a gene encoding a protein of the mannose-glucose-specific PTS and a glcK gene, 2) a gene encoding a protein of the mannose-glucose-specific PTS and a ccpA gene, and 3) a gene encoding a protein of the mannose-glucose-specific PTS, a glcK gene and a ccpA gene, wherein said strain, when used to ferment milk, provides a low lactose fermented milk and/or a fermented milk not undergoing acidification when stored at fermentation temperature.

The present invention relates to a lactic acid bacterium, in particular a strain of thegenus, with reduced sensitivity to cos-type bacteriophages. The invention also relates to methods to engineer these lactic acid bacteria as well as their use to ferment milk.

Lactic acid bacterium with a reduction of sensitivity to cos-type bacteriophages

Methods and compositions are provided for the use of anti-CRISPR (ACR) proteins in plants, including modulation of Cas endonuclease activity, improvement of frequency of homologous recombination, control of Cas endonuclease activity during various cell cycles, spatial and/or temporal regulation of Cas endonuclease activity in plants, usage in gene activation or repression, as well as reduction of off-target polynucleotide cleavage.

Anti-CRISPR polynucleotides and polypeptides and methods of use

The invention is directed to a lactose-positive, galactose-negative,strain releasing glucose during milk fermentation. Thisstrain carries mutation in the glcK gene, in the ccpA gene, in the lacZ gene and/or in the ptsH gene, and optionally in one or more genes, in particular one gene, encoding a protein of the mannose-glucose-specific PTS. The invention also concerns a composition comprising at least one, lactose-positive, galactose-negative,strain of the invention, and the use of this strain or composition to manufacture a fermented dairy product.

Lactic acid bacteria with sweetening properties and uses thereof

The invention relates to anti-CRISPR genes and anti-CRISPR proteins, and their uses in various biotechnology applications.

Anti-CRISPR genes and proteins and methods of use

The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof.

Method of modulating cell resistance

The present invention relates tostrains, usable as starter cultures, able to provide both satisfactory rheological and organoleptic properties, and satisfactory shelf life to the media into which they are incorporated. In particular, these strains are also bacteriophage-resistant, thereby minimizing bacteriophage infection. The invention also provides a composition comprising one of thesestrains, and feed or food products obtained with these strains.

strains

The disclosure relates in one aspect to a fast acidifying lactic acid bacterium that generates a viscosity in fermented milk greater than about 62 Pa&#xb7;s after 14 days of storage at 6&#xb0; C. Further, the disclosure includes method for preparing and products of food, food additive, feed, nutritional supplement, or probiotic supplement, thereof.

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The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.

Cultures with improved phage resistance

Method for modulating resistance

The present invention provides methods for tagging and/or identifying microorganisms. In some preferred embodiments, the microorganisms are bacteria. In some particularly preferred embodiments, the bacteria are members of the genus, while in other embodiments, the bacteria are members of other genera. The present invention also provides microorganisms tagged using the methods set forth herein. In some preferred embodiments, the tagged microorganisms are bacteria. In some particularly preferred embodiments, the tagged bacteria are members of the genus, while in other embodiments, the tagged bacteria are members of other genera.

Tagged microorganisms and methods of tagging

Growing community of inventors

Poitiers, France

Christophe Fremaux