Institut De Recherche Pour Le Developpement (ird)

Institut De Recherche Pour Le Developpement

Institut De L'environnement Et De Recherches Agricoles

Christophe Brugidou

Jean-Loup Lemesre

Alain Ghesquiere

Moumouni Konate

Drissa Sereme

Gnissa Konate

Christelle Sire

Denis Fargette

Jean-Paul Brizard

The invention relates to the use of genes which, in plants, encode proteins with a functional diversity in terms of silencing, comprising the selection of the gene with the level of effectiveness in order to construct a plant viral vector having the function of overexpressing or silencing particular genes.

Functional viral vectors for the overexpression or extinction of particular genes in plants, and applications thereof

The invention provides a composition for the inoculation in a plant of agrobacteria transfected by expression vectors, in order to produce in said plant a protein of interest or a derivative of said protein, by deletion or by mutation, characterized in that it comprises

Means for the transient production in plants of recombinant proteins that can be used in particular in prophylaxis and in therapeutics

The invention concerns a method for capturing the target proteins indispensable to the infectious cycles of a pathogenic virus, in particular the rice yellow mottle virus (RYMV) and for cloning the genes involved in said processes. The invention therefore concerns a method for identifying molecular markers of the resistance to RYMV. The method involves the isolation of said protein complexes with viral particles. The method consists in subjecting the samples containing said complexes to electrophoresis and Western Blot using a capsid anti-protein monoclonal antibody, and in recuperating the non-immunodetected bands. The invention also concerns a cDNA capable of being hybridized with a BAC (Bacterial Artificial Chromosome) screened from a bank consisting of DNA fragments of a variety of rice such as IR64. Said BAC clone contains DNA sequences of the markers identified from the rice by means of a process which consists in comparing the AFLP (Amplified Length Polymorphism) of resistant and sensitive rice plants.

Means for identifying a novel class of genes resistant to the rice yellow mottle virus and the locus of a major gene of resistance to the virus, and their applications

Growing community of inventors

Pignan, France

Christophe Brugidou