Carl Zeiss Meditec Ag

Carl Zeiss Microscopy Gmbh

Carl Zeiss Ag

Carl Zeiss Jena Gmbh

Carl Zeiss Microimaging Gmbh

Carl Zeiss Sports Optics Gmbh

Christoph Nieten

Enrico Geissler

Ingo Kleppe

Ralf Netz

Marco Pretorius

Guenter Rudolph

Yauheni Novikau

Bryce Anton Moffat

Daniel Bublitz

Marco Wilzbach

Artur Hoegele

Ralf Wolleschensky

Christoph Hauger

Alois Regensburger

Kai Wicker

Axel Krause

Alexander Gratzke

Markus Seesselberg

Roland Guckler

Nico Correns

Helge Jess

Tobias Breuninger

Thomas Mohr

Michael Rode

Norbert Kerwien

Michael Kieweg

Lars Omlor

Susanne Kohlhammer

Günter Rudolph

Michael Gölles

Artur Högele

Carsten Wehe

Stefan Meinkuss

Gunther Lorenz

Enrico Geißler

Enrico Geiβler

A microscopy system for simultaneously observing fluorescent and non-fluorescent regions of an object, and a method for operating the microscopy system are provided. The microscopy system includes a microscopy optical unit configured to image an object plane through an observation beam path onto an image plane, an observation filter arrangeable in the observation beam path, two light sources, one being provided for exciting a fluorescent dye in the object and another being provided for visualizing non-fluorescent regions of the object, and a controller to control the light sources individually. With a suitable configuration of the observation filter, all light sources can be operated with a minimum operating current, which ensures the stability of the light. The color rendering of the non-fluorescent regions can be set by the individual settability of the light sources which can be set such that fluorescent and non-fluorescent regions appear to be approximately equally bright.

Microscopy system and method for operating a microscopy system

A microscopy method is for producing an electronic image of an object, wherein the object is imaged with an adjustable optical imaging scale on an image detector. The method includes: selecting a parameter for the electronic image, wherein the parameter can be influenced by the optical imaging scale and differs from the image field dimensions, and setting a setpoint value range for the parameter, setting a total imaging scale for the electronic image, wherein adjusting or controlling the parameter of the electronic image is implemented such that, at the same time, the parameter of the electronic image lies in the specified setpoint value range with a tolerance and the set total imaging scale is obtained, wherein the optical imaging scale forms a basis for a manipulated variable of the adjustment or closed-loop control and a digital image magnification is carried out on the basis of the set total imaging scale.

Microscopy method and microscope for producing an image of an object

A microscope and method for high resolution scanning microscopy of a sample, having an illumination device, an imaging device for the purpose of scanning at least one point or linear spot across the sample and of imaging the point or linear spot into a diffraction-limited, static single image below a reproduction scale in a detection plane. A detector device is used for detecting the single image in the detection plane for various scan positions, with a location accuracy which, taking into account the reproduction scale in at least one dimension/measurement, is at least twice as high as a full width at half maximum of the diffraction-limited single image.

High-resolution scanning microscopy

A filter set for simultaneously observing fluorescent and non-fluorescent object regions, and an observation system and a method in which the filter set is used are disclosed. The illumination and observation light filters of the filter set each have two separate passbands with high transmission, which are spectrally defined such that illumination light outside the emission spectrum of fluorescence can pass through the illumination and observation light filters and fluorescent light is not swamped by the illumination light. The transmission of the passbands is defined such that non-fluorescent object regions can be observed substantially with color fidelity.

Filter set, system, and method for observing protoporphyrin IX

A microscopy method includes illuminating an object with illumination light, recording a first color image of the illuminated object by a color image sensor suitable for recording colors of a first gamut, producing a second color image of the object, the second color image including pixels that each have assigned a color from a second gamut, depicting the second color image by a display apparatus suitable for rendering colors of the second gamut, wherein the producing the second color image includes determining the colors at the pixels of the second color image by applying a color transfer function to the colors of the corresponding pixels of the first color image, the color transfer function mapping input colors onto output colors, and the color transfer function mapping those input colors that belong to the first gamut but not to the second gamut onto output colors that belong to the second gamut.

Microscopy method

In a projector for projecting a multi-colored image, a control unit actuates each color channel with reference to fed-in image data such that one of the color subframes of the multi-colored image to be projected is generated. A projection optical system images the generated color subframes onto a projection surface such that the color subframes can be perceived as the multi-colored image to be projected. Each color channel is formed for the generation of a color subframe of a predetermined base color. The color location of the predetermined base color varies with the lightness to be generated. The control unit controls each color channel such that, for at least one picture point in the multi-colored image, the color location shift of the predetermined target color location caused by the color channel or the color channels is compensated for while retaining the target lightness.

Projector for projecting a multi-colored image

The control unit of a projector drives modulators for the range of brightness of the image data with a first resolution of NN levels, wherein NN is an integer greater than one. The control unit applies a predefined brightness change to the brightness value in accordance with the image data for the image point such that a changed brightness value having a second resolution, which is greater than the first resolution, is calculated, and converts the changed brightness value into the increased brightness value such that it has the first resolution and is greater than a notional comparison value having the first resolution that arises if the predefined brightness change is applied to the brightness value in accordance with the image data, with the result that the control unit drives one of the modulators for a pixel to be boosted with the increased brightness value having the first resolution.

Projector for projecting images

An optical coherence tomograph includes a wavelength tunable illuminating device, an illumination and measurement beam path with a dividing element and a scanner and a front optical unit and a reference beam path, a detection beam path and a flat panel detector. A beam splitter conducts the separated measurement radiation to the detection beam path and an optical element acts only on the illumination radiation. The optical element sets the numerical aperture of the illumination of the illumination field in the eye. An optical element acts only on the measurement radiation and sets the numerical aperture with which measurement radiation is collected in the eye. An aperture is arranged in front of the flat panel detector in an intermediate image plane and defines the size of an object field. The flat panel detector has a spatial resolution of 4 to 100 pixels in a direction.

Optical coherence tomography for performing measurements on the retina

Microscope and method for high resolution scanning microscopy of a sample, wherein

An optical coherence tomograph that provides wavelength tunable source radiation and an illumination and measurement beam path, a dividing element that divides source radiation into illumination radiation and reference radiation, and collects measurement radiation. The illumination and measurement beam path has scanner. A detection beam path receives measurement radiation and reference radiation and conducts them onto at least one flat panel detector in a superposed manner. A beam splitter separates the measurement radiation from the illumination radiation. The beam splitter conducts the separated measurement radiation to the detection beam path and sets the numerical aperture of the illumination of the illumination field in the eye. An optical element sets the numerical aperture with which the measurement radiation is collected in the eye and a multi-perforated aperture defines the size of an object field and a number of object spots, from which the measurement radiation reaches the flat panel detector.

Optical coherence tomography for measurement on the retina

An optical detection filter has a transmission spectrum for detecting fluorescence light of a plurality of different fluorescent dyes. In the range between 350 nm and 1000 nm, the transmission spectrum has a first stopband (S1) from&#x3bb;1 to&#x3bb;2, a first passband (D1) from&#x3bb;2 to&#x3bb;3, a second stopband (S2) from&#x3bb;3 to&#x3bb;4, a second passband (D2) from&#x3bb;4 to&#x3bb;5, a third stopband (S3) from&#x3bb;5 to&#x3bb;6, and a third passband (D3) from&#x3bb;6 to&#x3bb;7. The stopbands (S1,S2,S3) each have a mean transmittance of at most 0.01, typically at most 0.001 or at most 0.0001, and the passbands (D1,D2) each have a mean transmittance of at least 0.5, typically at least 0.8 or at least 0.9; wherein 350 nm&#x2264;&#x3bb;1&#x3c;&#x3bb;2&#x3c;&#x3bb;3&#x3c;&#x3bb;4&#x3c;&#x3bb;5&#x3c;&#x3bb;6&#x3c;&#x3bb;7&#x3c;1000 nm.

Optical filter system and fluorescence detection system

An imaging system with an imaging lens system for imaging an object into an image plane is disclosed. The imaging lens system contains an optical component for a higher depth of field, of which the refractive power is alterable and the optical effect remains rotation-symmetrical.

Imaging system and imaging method

A method for operating a scanning microscope and for determining point spread functions, with which sample images are recorded with the scanning microscope. The method includes scanning a sample with at least one illuminating light beam; recording at least one sample image with a detector device during a scan by the illuminating light beam; and comprising the point spread function, with which a sample image is recorded, from the at least one sample image. A detector device having receiving elements is used, where the distance between the receiving elements is smaller than a diffraction disk that generates a sample point on the detector device. Detector signals, generated by means of the receiving elements, are read out for each of the different positions of the illuminating light beam on the sample, as a result of which the scanning of the sample allows the detector signals, which are read out, to generate a plurality of sample images. In this case the point spread functions with respect to the different detector signals are defined in each instance by means of an illumination point spread function and a detection point spread function. With respect to all of the detector signals, a matching illumination point spread function is assumed that is shifted in accordance with the scanning motion for different detector signals. In addition, with respect to all of the detector signals, a matching detection point spread function is assumed that takes account of a spatial offset between the detector elements. The plurality of sample images are used to compute the illumination point spread function and the detection point spread function, and these functions are used to compute the point spread functions with respect to the different detector signals. In addition, the invention also relates to a corresponding scanning microscope.

Scanning microscope and method for determining the point spread function (PSF) of a scanning microscope

In a microscope for high resolution scanning microscopy of a sample, said microscope comprising&#x2014;an illumination device for illuminating the sample, &#x2014;an imaging device for scanning at least one point spot or line spot across the sample and for imaging the point spot or line spot into a diffraction-limited, stationary single image with magnification into a detection plane, &#x2014;a detector device for detecting the single image in the detection plane for different scanning positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited single image, &#x2014;an evaluation device for evaluating a diffraction pattern of the single image for the scanning positions from data of the detector device and for generating an image of the sample, said image having a resolution that is increased beyond the diffraction limit, provision is made for&#x2014;the detector device to have a detector array, which has pixels and is larger than the single image, and&#x2014;a non-imaging redistribution element, which is disposed upstream of the detector array and distributes the radiation from the detection plane in a non-imaging manner among the pixels of the detector array.

Growing community of inventors

Jena, Germany

Christoph Nieten