Massachusetts Institute of Technology

Harvard College

Children's Medical Center Corporation

The Broad Institute, Inc.

Christoph Muus

Aviv Regev

Vijay G Sankaran

Jason Daniel Buenrostro

Leif S Ludwig

Jacob C Ulirsch

Caleb A Lareau

Embodiments disclosed herein provide methods of using somatic mutations in mitochondrial genomes to retrospectively infer cell lineages in native contexts and to serve as genetic barcodes to measure clonal dynamics in complex cellular populations. Further, somatic mutations in mitochondrial DNA (mtDNA) are tracked by single cell genomic approaches for simultaneous analysis of single cell lineage and state. Applicants further show that mitochondrial mutations can be readily detected with contemporary single cell transcriptomic and epigenomic technologies to concomitantly capture gene expression profiles and chromatin accessibility, respectively.

Lineage tracing using mitochondrial genome mutations and single cell genomics

Growing community of inventors

Cambridge, MA, United States of America

Christoph Muus