Leica Microsystems Cms Gmbh

Christoph Greb

Falk Schlaudraff

Florian Hoffmann

A method for obtaining dissectates from a microscopic sample using a laser microdissection system having a laser unit includes: a) at least partially circumcising and releasing from the sample dissectate regions of the sample as the dissectates using laser pulses provided by the laser unit; b) transferring the dissectates, by being released from the sample, along dissectate trajectories into receptacles of a dissectate collection unit; and c) positioning the receptacles of a dissectate collection unit using a positioning unit to collect the dissectates. The positioning of the receptacles of the dissectate collection unit using the positioning unit is automatically performed based on estimates of the dissectate trajectories, the estimates of the dissectate trajectories being obtained in a learning mode, the learning mode including obtaining dissectates by repeatedly performing at least steps a) and b). Parameters of the dissectate trajectories are determined for a plurality of dissectates.

Method for obtaining dissectates microscopic sample, laser microdissection system and computer program

A method for checking a dissection process in a laser microdissection system includes carrying out the dissection process for cutting out a dissectate from an object in a first region of the object by a laser beam. First image data is acquired of at least the first region of the object after the dissection process. It is examined whether the first image data has sharp structures within a region to be separated by the dissection process in order to determine whether the dissection process was successful.

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Marburg, Germany

Christoph Greb