Other

Bruker Daltonik Gmbh

Christoph Gebhardt

Ralph Kikillus

Andreas Brekenfeld

Ralf Hartmer

Peter Aagaard Nielsen

Nicolai Bache

Desmond Allen Kaplan

Frank Hirmer

Ole Bjeld Hoerning

Michael Duerr

Christian Meier-Credo

Peter A Nielsen

The invention relates to a liquid junction apparatus for electrospray ionization in a mass spectrometer comprising an electrospray emitter, a capillary conduit assembly for conducting liquid to be electrosprayed, and a union comprising an electrically conductive material, in which the electrospray emitter and the capillary conduit assembly are accommodated in a bottom-sealing butt joint featuring low dead volume while retaining at least one of them pluggable and withdrawable. The liquid junction apparatus facilitates energizing the transmitted liquid to a predetermined voltage level at the liquid junction upstream of an actual emitter tip where electrospraying occurs, while retaining at least one of an electrospray emitter and a capillary conduit assembly pluggable into and withdrawable from a union that comprises a conductive material.

Liquid junction apparatus for electrospray ionization

A low dead-volume connector for fluid chromatography, such as liquid chromatography and gas chromatography, has a female assembly including a receiving member being designed and configured to receive, and releasably interlock with, a male assembly. The connector is particularly suited for high performance liquid chromatography (HPLC) applications.

Low dead-volume connector for fluid chromatography

The invention generally relates to a connector for fluid chromatography, such as gas chromatography and liquid chromatography, having a female assembly including a receiving member being designed and configured to receive, and releasably interlock with, a male assembly. The connector is particularly suited for high performance liquid chromatography (HPLC) applications.

Connector for fluid chromatography

The invention relates to devices and methods in mass spectrometers for the generation of ions of heavy molecules, especially biomolecules, by bombarding them with uncharged clusters of molecules. The analyte ions which are generated or released by cluster bombardment of analyte substances on the surface of sample support plates show a broad distribution of their kinetic energies, which prevents good ion-optical focusing. In the invention, the kinetic energies are homogenized in a higher-density collision gas. The collision gas is preferably located in an RF ion guide, more preferably an RF ion funnel, which can transfer the ions to the mass analyzer. The collision gas may be introduced with temporal pulsing, coordinated or synchronized with the pulsed supersonic gas jet. The collision gas may be pumped off again before the next supersonic gas pulse. In an advantageous embodiment, the collision gas can originate from the supersonic gas jet itself.

Ion generation in mass spectrometers by cluster bombardment

The invention proposes a method for the collective ejection of ions from a 3D RF ion trap with a ring electrode and two end cap electrodes, which comprises the following steps: (a) the RF voltage of a high-quality resonant circuit applied to the ring electrode is replaced with a second RF voltage at the two end cap electrodes which can be changed or switched faster than the high voltage at the ring electrode, keeping the ions stored, (b) the second RF voltage at the end cap electrodes is then switched down or off abruptly, releasing the ions, and (c) the released ions are ejected through an opening in one of the end cap electrodes by switching on a DC voltage on at least one of the end cap electrodes.

Ejection of ion clouds from 3D RF ion traps

In an RF quadrupole ion trap having electrodes to which RF voltages are applied, ions having m/z ratios outside of a predefined narrow range of charge-related masses m/z are removed from the trap by applying a DC voltage pulse to at least one of the trap electrodes to remove from the trap the ions with high values of charge-related masses. The DC voltage pulse is preferably applied in combination with a variation of the RF voltage amplitudes to simultaneously remove from the trap ions of low charge-related masses. The DC and RF voltage amplitudes are changed in such a manner that any excitation of ions having charge-related masses within the predefined range by frequency mixtures is avoided.

Growing community of inventors

Bremen, Germany

Christoph Gebhardt