Albert-ludwigs-universitaet Freiburg

Albert-ludwigs-universitat Freiburg

Helmholtz-zentrum Fur Umweltforschung Gmbh-ufz

Christine Reinemann

Guenter Roth

Beate Strehlitz

Regina Stoltenburg

Günther Roth

The invention relates to a method for identifying aptamers, having the following steps &#x2014;bringing a mixture of oligonucleotides into contact with an aptamer target structure and binding at least some of the oligonucleotides to the target structure, &#x2014;separating the oligonucleotides which have been bound to the aptamer target structure from the aptamer target structure and from oligonucleotides that are not bound to the aptamer target structure, &#x2014;amplifying individual oligonucleotides which were bound to the aptamer target structure in a physically separate manner and producing a plurality of physically separate amplicons, each amplicon predominantly containing one type of oligonucleotides, &#x2014;specifying a specific marker for a plurality of the physically separate amplicons such that each of the marked amplicons can be uniquely identified using the specified marker of the amplicon, &#x2014;sequencing oligonucleotides in a plurality of marked amplicons and assigning the marker that is specific for the amplicon to the sequence of the type of oligonucleotides in the amplicon for each amplicon examined by means of the sequencing process &#x2014;analyzing the binding properties of the types of oligonucleotides to the aptamer target structure and assigning the analyzed binding properties to the specific markers of the amplicons and to the sequences of the types of oligonucleotides.

Growing community of inventors

Leipzig, Germany

Christine Reinemann