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Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur

Institut Pasteur and Centre National De La Recherche Scientifique

Christine Pourcel

Pierre Tiollais

Patrick Charnay

Charles Chany

Alex Fritsch

Anne Louise

Francois Galibert

Marie-Francedillaoise Dubois

Marie-Francoise Dubois

A process produces DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. Vectors contain the cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B. Nucleic acid of reduced size and a vector containing the nucleotide sequence of which DNA codes an immunogenic peptide sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 9A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in the serum.

NUCLEOTIDE SEQUENCE COMPRISING THE GENOME OF HEPATITIS B VIRUS, NUCLEOTIDE SEQUENCE CODING THE SURFACE ANTIGEN OF THE HEPATITIS B VIRUS, VECTORS CONTAINING THE NUCLEOTIDE SEQUENCES, PROCESS FOR THE PREPARATION THEREOF, AND ANTIGEN OBTAINED THEREBY

The invention relates to a process for producing DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. The invention also relates to vectors containing said cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B.

Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it

Method for the production of antigens vaccinating against the virus of B viral hepatitis. It consists of transforming a cell culture with a vector, more particularly a plasmid, itself containing an insertion sequence including itself at least the part of the viral DNA coding for the immunogen protein, capable of inducing in vivo antibody production active with respect to the whole virus, as well as the viral promoter under the control of which the transcription and translation of the above-said part of viral DNA is normally carried out, in particular in a host infected with the corresponding virus.

Method for the transformation of cells, particularly eukaryotes by a DNA

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Paris, France

Christine Pourcel