Apceth Gmbh & Co Kg

Junctucell Biomed Manufacturing Gmbh

Martin-Luther-Universität Halle-Wittenberg

Christine Günther

Ralf Huss

Felix Hermann

Stefanos Theoharis

Thomas Müller

Elena Aseeva

Ulf Geumann

Manfred Stangl

Daria Larissa Forster

Sabine Geiger-schredelseker

A method of treating a tumor in a subject including administering a genetically modified mesenchymal stem cell (MSC), wherein the MSC includes one or more exogenous nucleic acid molecule(s), wherein the one or more exogenous nucleic acid molecule(s) includes one or more regions encoding two or more immune response-stimulating cytokines operably linked to one or more promoters or promoter/enhancer combinations, wherein the two or more immune response-stimulating cytokines include at least IL-7, and at least one of IL-12 or IL-21.

Genetically modified mesenchymal stem cells expressing an immune response-stimulating cytokine to attract and/or activate immune cells

This invention provides a cell growth medium comprising (a) a human platelet lysate free of solid matter greater than 0.22 &#x3bc;m in diameter, wherein the lysate constitutes from 2% to 15% of the total volume of the cell growth medium; (b) a human fresh frozen plasma (FFP) filtrate free of solid matter greater than 0.22 &#x3bc;m in diameter, wherein the FFP filtrate constitutes from 1% to 10% of the total volume of the cell growth medium; (c) heparin at a concentration of from 0 U/ml to 10 U/ml of the cell growth medium; (d) L-glutamine at a concentration of from 0.5 mM to 10 mM; and (e) a serum-free, low glucose medium suitable for mammalian cell growth, wherein the serum-free, low glucose medium constitutes from 75% to 97% of the total volume of the cell growth medium, and may contain the L-glutamine of part (d); wherein the cell growth medium permits the expansion of human CD34stem cells and wherein the resulting expanded CD34stem cells retain the ability to differentiate. This invention also provides related cell growth medium supplements, a sterile human platelet lysate and human fresh frozen plasma (FFP) filtrate, kits, CD34stem cell-containing compositions, and related production and cell expansion methods.

Expansion medium for CD34-negative stem cells

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Munich, Germany

Christine Günther