Dharmacon, Inc.

Ge Healthcare Dharmacon, Inc.

Christina Yamada

Anastasia Khvorova

Devin Leake

Rob Kaiser

Emily Anderson

Annaleen Vermeulen

Barbara Robertson

William Marshall

James R Ladine

Scott Baskerville

Yuriy Fedorov

Provided herein are tripartite oligonucleotide complexes which can be administered to a cell, tissue or organism to silence a target gene. The tripartite oligonucleotide complexes of the disclosure may include a conjugate moiety that facilitates delivery to a cell, tissue or organism without the aid of a transfection reagent.

Tripartite oligonucleotide complexes and methods for gene silencing by RNA interference

Provided herein are duplex oligonucleotide complexes which can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent(s). The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue or organism.

Duplex oligonucleotide complexes and methods for gene silencing by RNA interference

Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.

Duplex oligonucleotides with enhanced functionality in gene regulation

Compositions, kits, systems, equipment, and protocols utilize synthetic siRNA having a delivery facilitating moiety in improved bioprocesses that enhance the production of biomaterials. The siRNA can target genes associated with the following: 1) deleterious vector derived genes; 2) genes that confer non-optimal growth or differentiation properties to the cells; 3) genes that can influence heterogeneity or post-translational modification pattern of the desirable gene product; 4) genes that highly express non-desired proteins; 5) genes that express proteins which interfere with purification of the desired protein; and 6) other genes that can interfere with the bioprocess.

Enhanced biotherapeutic production using inhibitory RNA

Growing community of inventors

Boulder, CO, United States of America

Christina Yamada