Wisconsin Alumni Research Foundation

Christina L Pier

Eric Arthur Johnson

William Howard Tepp

Marite Bradshaw

Joseph T Barbieri

Michael Baldwin

A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparingtransformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.

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Fitchburg, WI, United States of America

Christina L Pier