Fujifilm Cellular Dynamics, Inc.

Fujifilm Holdings America Corporation

Christie Munn

Deepika Rajesh

Sarah Burton

Bradley Swanson

Maksym A Vodyanyk

Wen Bo Wang

Xin Zhang

Andrew J Brandl

Makiko Ohshima

Anne Strouse

Katherine Czysz

Ethan McLEOD

Madelyn Donegan

Igor Gurevich

The present disclosure provides methods of producing hepatocytes from induced pluripotent stem cells. Further provided herein are methods of using the hepatocytes for the treatment of a liver disease.

Methods for the production of hepatocytes

Provided herein are methods for the production of antigen-specific effector T cells and NK cells from pluripotent stem cells which express a chimeric antigen receptor (CAR). Further provided herein are methods for the adoptive cell therapy by administering the effector T cells and/or NK cells provided herein.

Antigen-specific immune effector cells

Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Methods for directed differentiation of pluripotent stem cells to immune cells

Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.

Generating mature lineages from induced pluripotent stem cells with MECP2 disruption

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Madison, WI, United States of America

Christie Munn