Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Abberior Instruments Gmbh

Christian Wurm

Stefan W Hell

Vladimir Belov

Gerald Donnert

Lars Kastrup

Benjamin Harke

Kirill Kolmakov

Stefan Jakobs

Christian Eggeling

Andreas Schoenle

Katrin Willig

Shamil Nizamov

Heiko Schill

Volker Westphal

Matthias Reuss

Mariano Bossi

Andreas Schönle

Johanna Wildanger

Alexey Butkevich

Gyuzel Mitronova

Gael Moneron

Maksim Sednev

Heydar Shojaei

Jakob Bierwagen

Lars Meyer

Dirk Kamin

Vadim P Boyarskiy

Claudia Geisler

Marcel Lauterbach

Christian Ringemann

Vladimir N Belov

Jörn Heine

The invention is directed to a method for recording a motion trajectory of an individual particle in a sample and to a light microscope performing this method. Starting from an at least approximately known initial position, the particle is scanned with an intensity distribution of a scanning light comprising a local intensity minimum. When illuminated with the scanning light, the particle to be tracked generates a detectable light signal, from the intensity of which updated coordinates of the particle are calculated. According to the invention, the scanning is terminated when a second measured variable detected in parallel satisfies a termination criterion.

Method and microscope for recording trajectories of individual particles in a sample

In methods of high-resolution imaging a structure of a sample, the structure being marked with fluorescence markers, the sample is subjected to a light intensity distribution including an intensity maximum of focused fluorescence excitation light to selectively scan partial areas of interest of the sample. Fluorescence light emitted out of the sample is registered and allocated to a respective location of the light intensity distribution in the sample. The subjection of the sample to at least one part of the light intensity distribution is terminated at each location of the light intensity distribution, if at least one criterion of the following criteria is met: (a) a predetermined maximum light amount of the fluorescence light emitted out of the sample has been registered, and (b) a predetermined minimum light amount of the fluorescence light emitted out of the sample has not been registered within a predetermined period of time.

Methods of high-resolution imaging a structure of a sample, the structure being marked with fluorescence markers

Compounds of formula I are disclosed: wherein X, X, X, Xare independently H, F, Cl, Br, I, CN, NO, OR, SR, NRR, COR, COOR, CONRR, PORR, SOR, SORor R; Rand Rare, e.g., H, alkyl or aryl or optionally a ring; Ris, e.g., alkyl, alkenyl, alkynyl, aryl or cycloalkyl; Y is OR, NRR, or NRR; Q is O, S, SO, NR, C(R), Si(R), Ge(R), P(&#x2550;O)Ror P(&#x2550;O)OR; Q and Xcan optionally form part of a ring; L and M are independently OR, SR, NRRand R; L and M can optionally form part of a ring; Z is O, S, NR, CRRor aryl; and Z and Xcan optionally form part of a ring.

Dyes with phosphinic acid, phosphinate, phosphonate and phosphonamidate substituents as auxochromic groups and methods for preparing the same

The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O)YYor P(O)YY, where Yand Yindependently denote any of the following residues: OH, O(&#x2212;), ORand OR, NHRand NHR, NRRand NRR, ORand NHR, ORand NRR, NHRand NRR; and any salt thereof.

Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques

The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.

Fluorinated rhodamines as photostable fluorescent dyes for labelling and imaging techniques

The present invention relates to novel photoactivable rhodamine or carbopyronine derivatives of the following general formulae G1-G4 (I), G1: A1=O, A2=N, A3=C; G2: A1=S, A2=N, A3=C; G3: A1=O, A2=O, A3=N; G4: A1=S, A2=O, A3=N; comprising UV light absorbing chromophores which after photolysis by irradiation at 254-490 nm (preferably at 375-420 nm) generate fluorescent rhodamine or carbopyronine derivatives and small non-toxic fragments such as Nor NO. The invention also relates to methods for preparing such compounds and to the use of these compounds for optical microscopy and imaging techniques. 

Photoactivable fluorescent dyes for optical microscopy and imaging techniques

The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH, C&#x2550;O, C&#x2550;NOR, C&#x2550;NNRNR, CH(OR), O, S, SO, SO, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion. 

Hydrophilic and lipophilic rhodamines for labelling and imaging

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Goettingen, Germany

Christian Wurm